Polymer surface reorientation after protein adsorption
Polymer surface reorientation after protein adsorption
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DOI：
10.1021/la0607656
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发表时间：
2006-10-10
期刊：
影响因子：
3.9
通讯作者：
Chen, Zhan
中科院分区：
文献类型：
--
作者：
Clarke, Matthew L.;Chen, Zhan
用和频产生振动光谱法观察了两种聚合物在蛋白质吸附过程中表面侧链取向的变化。侧链氘化聚（甲基丙烯酸乙酯）和聚（甲基丙烯酸正丁酯）与五种蛋白质溶液接触：白蛋白、纤维蛋白原、泛素、细胞色素c和溶菌酶。与这些不同介质接触的表面聚合物侧链的CD3/CD2对称拉伸比相互比较，并与与空气或磷酸盐缓冲盐水接触的聚合物的CD3/CD2对称拉伸比进行比较。不同蛋白质在表面的吸附导致聚合物侧链的取向略有不同，取向在空气和缓冲壳之间。
Surface side-chain orientation changes of two polymers have been observed upon protein adsorption using sum frequency generation vibrational spectroscopy. Side-chain-deuterated poly( ethyl methacrylate) and poly(n-butyl methacrylate) were contacted with five protein solutions: albumin, fibrinogen, ubiquitin, cytochrome c, and lysozyme. The CD3/CD2 symmetric stretch ratios of the surface polymer side chains in contact with these different media were compared to each other and to that of the polymer contacting air or phosphate buffered saline. The adsorption of different proteins to the surfaces resulted in polymer side-chain orientations slightly different from each other, with orientations between the air and buffer cases.