A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY
A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY
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DOI：
10.1016/0006-2952(61)90145-9
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发表时间：
1961-01-01
影响因子：
5.8
通讯作者：
FEATHERSTONE, RM
中科院分区：
文献类型：
--
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
一种用于测定组织提取物、匀浆、细胞悬液等的乙酰胆碱酯酶活性的光度法，已经被描述了。通过跟踪当硫代胆碱与二硫代双硝基苯甲酸根离子反应时由硫代胆碱产生的黄色的增加来测量酶活性。它基于这些反应的耦合：后一反应是快速的，并且测定是灵敏的（即10 μ1血液样品是足够的）。录音机的使用是最有帮助的，但不是必需的。该方法已用于研究人红细胞和大鼠脑、肾、肺、肝和肌肉组织匀浆中的酶。用该体系测定了红细胞胆碱酯酶的动力学常数。用乙酰硫代胆碱作为底物得到的数据与用乙酰胆碱得到的数据相似。
A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described. The enzyme activity is measured by following the increase of yellow color produced from thiocholine when it reacts with dithiobisnitrobenzoate ion. It is based on coupling of these reactions: The latter reaction is rapid and the assay is sensitive (i.e. a 10 μ1 sample of blood is adequate). The use of a recorder has been most helpful, but is not essential. The method has been used to study the enzyme in human erythrocytes and homogenates of rat brain, kidney, lungs, liver and muscle tissue. Kinetic constants determined by this system for erythrocyte eholinesterase are presented. The data obtained with acetylthiocholine as substrate are similar to those with acetylcholine.