Potentiometric study of cytochrome c1aa3 from Thermus thermophilus.
Potentiometric study of cytochrome c1aa3 from Thermus thermophilus.
复制标题
嗜热栖热菌细胞色素 c1aa3 的电位研究。
DOI:
10.1016/0162-0134(85)85036-4
复制
发表时间:
1985
影响因子:
3.9
通讯作者:
Fee,JA
中科院分区:
文献类型:
--
作者:
Yoshida,T;Fee,JA
We have examined the redox behavior of the cytochromec1aa3complex fromThermus thermophilus. In potentiometric titrations the cytochromecbehaves as an independent center havingn= 1 andE= 205 mV (NHE). Under the assumption that the individual centers equilibrate independently in this experiment, changes in the absorption band at 603 nm have been resolved into two components: cytochromea(n= 1,Em= 270 mV, 60% spectral contribution) and cytochromea3(n= 2,Em= 360 mV, 40% spectral contribution). Then= 2 process was attributed to strong chemical coupling between cytochromea3and CuB. The enzyme was also titrated with a mixture of NADH and PMS, and the results are shown not to conform to a model of intramolecular equilibrium according to the equilibrium constants obtained from the potentiometric titration. It is suggested that a conformational equilibrium within the complex may control electron transfer between cytochromesaanda3.
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DOI:
--
发表时间:
1981
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Bull,C;Ballou,DP;Otsuka,S
通讯作者:
Otsuka,S
DOI:
--
发表时间:
1982
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Antalis,TM;Palmer,G
通讯作者:
Palmer,G
影响因子:
3.4
作者:
Powers,L;Chance,B;Ching,Y;Angiolillo,P
通讯作者:
Angiolillo,P
DOI:
--
发表时间:
1964
期刊:
影响因子:
--
作者:
B. F. Gelder;A. Muijsers
通讯作者:
A. Muijsers
DOI:
--
发表时间:
1981
期刊:
影响因子:
--
作者:
M. Wikström;K. Krab;M. Saraste
通讯作者:
M. Saraste