Changes in the immunogold electron-microscopic localization of calpain in bovine skeletal muscle induced by conditioning and high-pressure treatment
Changes in the immunogold electron-microscopic localization of calpain in bovine skeletal muscle induced by conditioning and high-pressure treatment
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DOI：
10.1271/bbb.70.1249
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发表时间：
2006-05-01
影响因子：
1.6
通讯作者：
Suzuki, Atsushi
中科院分区：
文献类型：
--
作者：
Borjigin, Gerelt;Yamamoto, Shuhei;Suzuki, Atsushi
用免疫金电子显微镜研究了加压和条件处理后牛骨骼肌钙蛋白酶的变化。在解冻后即刻的肌肉中（对照），通过计数胶体颗粒统计计算的胶体颗粒的相对分布分别约为65%和35%在I带/Z盘和A带区域。在肌肉调理7天，胶体颗粒的分布是两个以上的两倍以上的I-带/Z-盘和A-带区域比对照肌肉。在进一步储存期间，几乎没有检测到胶体颗粒的绝对浓度或相对分布的变化。在暴露于100 MPa或200 MPa的压力的肌肉中，在肌节中检测到比对照肌肉中略多的免疫金。将压力增加到300 MPa，可以在整个肌节中看到高密度颗粒。相反，在暴露于400 MPa的肌肉的肌节中检测到很少的颗粒。这些电子显微镜观察结果从统计分析中得到证实，与条件肌肉一样。从所获得的结果中可以清楚地看出，压力诱导的钙蛋白酶定位变化比条件反射引起的变化明显得多。
Changes in the localization of calpain in conditioned and pressurized bovine skeletal muscles were investigated by immunogold electron-microscopy. In the muscle immediately after thawing (control), the relative distributions of colloidal particles statistically calculated by counting the colloidal particles were about 65% and 35% in the I-band/Z-disk and A-band regions, respectively. In the muscle conditioned for 7 days, distribution of colloidal particles was more than two times greater in both the I-band/Z-disk and A-band regions than in the control muscle. Almost no change in either the absolute concentration or relative distribution of the colloidal particles was detectable during further storage. In the muscle exposed to a pressure of 100 MPa or, 200 MPa, slightly more immunogold was detected in the sarcomere than in that of the control muscle. Increasing pressure up to 300 MPa enabled high-density particles to be seen throughout the sarcomere. Conversely, few particles were detected in the sarcomere of the muscle exposed to 400 MPa. These electron-microscopic observations were confirmed from the statistical analysis as with the conditioned muscles. It was clear from the results obtained that the pressure-induced changes in calpain localization were much;more pronounced than those from conditioning.