Serial analysis of gene expression in human monocytes and macrophages
Serial analysis of gene expression in human monocytes and macrophages
复制标题
DOI：
10.1182/blood.v94.3.837.413k02_837_844
复制
发表时间：
1999-08-01
期刊：
影响因子：
20.3
通讯作者：
Matsushima, K
中科院分区：
文献类型：
--
作者：
Hashimoto, S;Suzuki, T;Matsushima, K
单核/巨噬细胞作为哨兵参与慢性炎症和各种病原体的根除。为了从分子上定义血液单核细胞向巨噬细胞的分化，我们对粒细胞-巨噬细胞集落刺激因子（GM-CSF）或M-CSF诱导的人血液单核/巨噬细胞进行了一系列基因表达分析（SAGE）， SAGE分别分析了来自单核细胞、GM-CSF-和M-CSF诱导的巨噬细胞的57,560、57,463和55,856个标签，鉴定出35,037个不同的转录本。有趣的是，在单核细胞向巨噬细胞分化的过程中表达最高的基因是参与脂质代谢的基因，两种csf诱导的巨噬细胞表达相似的基因组，除了一些基因，如单核细胞衍生趋化因子（MDC）、豆类蛋白、前列腺素D合成酶和溶酶体唾液糖蛋白。或m - csf诱导的巨噬细胞提供了新的方法来定义巨噬细胞亚群和巨噬细胞谱系细胞的成熟和激活阶段，并可能诊断巨噬细胞发挥主要作用的疾病。这项研究代表了对任何类型的人类造血细胞的基因表达的第一次广泛的系列分析。(C) 1999年由美国血液病学会出版。
Monocytes/macrophages serve as sentinels involved in chronic inflammation and the eradication of various pathogens. To define molecularly the differentiation of blood monocytes into macrophages, we conducted serial analysis of gene expression (SAGE) in human blood monocytes/macrophages induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) or M-CSF, SAGE analysis of 57,560, 57,463, and 55,856 tags from monocytes, GM-CSF-, and M-CSF-induced macrophages, respectively, allowed identification of 35,037 different transcripts. Interestingly, the genes with the highest expression during differentiation from monocytes into macrophages were genes involved in lipid metabolism, Both CSF-induced macrophages ex-pressed similar sets of genes except for several genes such as monocyte-derived chemokine (MDC), legumain, prostaglandin D synthetase, and lysosomal sialoglycoprotein, The identification of specific gene expression in human monocytes, GM-CSF-, or M-CSF-induced macrophages provides novel methods to define macrophage subsets and the maturation and activation stage of cells of macrophage lineage and, possibly, to diagnose diseases in which macrophages play a major role, This study represents the first extensive serial analysis of gene expression for any type of human hematopoietic cells. (C) 1999 by The American Society of Hematology.