Biodegradation of acephate and methamidophos by a soil bacterium Pseudomonas aeruginosa strain Is-6
Biodegradation of acephate and methamidophos by a soil bacterium Pseudomonas aeruginosa strain Is-6
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DOI：
10.1080/03601234.2013.836868
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发表时间：
2014-01-02
影响因子：
2
通讯作者：
Seetharaman, Barathi
中科院分区：
文献类型：
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作者：
Ramu, Sasikala;Seetharaman, Barathi
从农田土壤中分离鉴定一株新的甲胺磷降解菌，并对其降解能力和降解途径进行研究。从农田土壤中分离到一株能完全降解乙酰甲胺磷的菌株Is-6，该菌株在M9培养基上生长时能以乙酰甲胺磷为唯一碳源、磷源和能源。16 S rRNA基因序列分析和表型分析表明，菌株Is-6属于铜绿假单胞菌（Pseudomonas aeruginosa）。通过高效液相色谱（HPLC）和电喷雾质谱（ESI-MS）分析，菌株Is-6能在96 h内完全降解乙酰甲胺磷（50 mg L-1）及其代谢产物。在较高浓度下，菌株Is-6对乙酰甲胺磷（1000 mg·L ~（-1））的降解率为92%。对乐果、敌百虫、甲基敌百虫、毒死蜱、马拉硫磷也有一定的利用价值。菌株Is-6（10（7）cells g（-1））接种到乙酰甲胺磷（50 mg Kg（-1））处理的土壤中，其降解率高于未接种的土壤。这些结果突出了这种细菌用于清理环境中受污染的农药废物的潜力。
The aim of this study was to isolate and characterize a new acephate-degrading bacteria from agricultural soil and to investigate its biodegradation ability and pathway of degradation. A bacterial strain Is-6, isolated from agriculture soil could completely degrade and utilize acephate as the sole carbon, phosphorus and energy sources for growth in M9 medium. Analysis of the 16S rRNA gene sequence and phenotypic analysis suggested that the strain Is-6 was belonging to the genus Pseudomonas aeruginosa. Strain Is-6 could completely degrade acephate (50mg L-1) and its metabolites within 96h were identified by high-performance liquid chromatography (HPLC) and electron spray ionization-mass spectrometry (ESI-MS) analyses. When exposed to the higher concentration, the strain Is-6 showed 92% degradation of acephate (1000mg L-1) within 7days of incubation. It could also utilize dimethoate, parathion, methyl parathion, chlorpyrifos and malathion. The inoculation of strain Is-6 (10(7) cells g(-1)) to acephate (50mg Kg(-1))-treated soil resulted in higher degradation rate than in noninoculated soils. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.