Effects of D-glucosamine, D-mannosamine, and 2-deoxy-D-glucose on the ultrastructure of ascites tumor cells in vitro.
Effects of D-glucosamine, D-mannosamine, and 2-deoxy-D-glucose on the ultrastructure of ascites tumor cells in vitro.
复制标题
D-葡萄糖胺、D-甘露糖胺和2-脱氧-D-葡萄糖对体外腹水肿瘤细胞超微结构的影响。
作者：
Z. Molnar;J. Bekesi
用氨基糖处理体外培养的埃利希腹水癌和Sarcoma 180腹水癌细胞，用光镜和电镜观察其变化。此外，d-葡糖胺或d-甘露糖胺的孵育介质引起显着的细胞质和核的变化。最早的变化，孵育15分钟后，看到的，包括空泡化的细胞质和分离的电子透明丝状部分的核仁和核仁空泡在核仁的周边。随着孵育时间的延长，细胞质空泡化程度逐渐增加，并伴有细胞质向核周围收缩。电子透明成分完全挤出后，核仁变得致密。在孵育3小时后，95%的肿瘤细胞有致密的，多态性的，或崩解的核，和几乎所有的细胞检查的核仁几乎完全由紧凑的，颗粒状的，电子致密的核仁线。4小时后，大多数肿瘤细胞表现出不同程度的崩解。这些肿瘤细胞系与2-脱氧葡萄糖孵育4小时，导致腹水肿瘤细胞中没有显着的结构改变。
Ehrlich ascites carcinoma and Sarcoma 180 ascites tumor cells were treated in vitro with amino sugars, and the resulting changes were studied by light and electron microscopy. Addition of d-glucosamine or d-mannosamine to the incubation medium provoked striking cytoplasmic and nuclear changes. The earliest changes, seen after incubation for 15 min, included vacuolization of the cytoplasm and separation of the electron-lucent filamentous parts of the nucleolonema and nucleolar vacuole at the periphery of the nucleolus. As the time of incubation increased, vacuolization of the cytoplasm increased gradually, accompanied by retraction of the cytoplasm around the nucleus. After complete extrusion of its electron-lucent components, the nucleolus became condensed. At the end of 3 hr of incubation, 95% of the tumor cells had pycnotic, polymorphic, or disintegrating nuclei, and the nucleoli in nearly all the cells examined consisted almost exclusively of the compacted, granular, electron-dense nucleolonema. After 4 hr, most tumor cells exhibited various degrees of disintegration. Incubation of these tumor cell lines for 4 hr with 2-deoxyglucose resulted in no significant structural alteration in the ascites tumor cells.