Late and persistent up-regulation of intercellular adhesion molecule-1 (ICAM-1) expression by ionizing radiation in human endothelial cells in vitro
Late and persistent up-regulation of intercellular adhesion molecule-1 (ICAM-1) expression by ionizing radiation in human endothelial cells in vitro
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DOI：
10.1080/095530097143428
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发表时间：
1997-08-01
影响因子：
2.6
通讯作者：
Mouthon, MA
中科院分区：
文献类型：
--
作者：
Gaugler, MH;Squiban, C;Mouthon, MA
黏附分子在细胞通过血管内皮细胞的运输中起关键作用，特别是在白细胞从血液迁移到组织中的炎症反应过程中。鉴于炎症是辐射损伤的主要后果之一，我们研究了电离辐射对人脐静脉内皮细胞(HUVEC)细胞表面细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)和E-选择素表达的影响。流式细胞仪检测显示，照射后的HUVEC ICAM-1的基础表达呈时间和剂量依赖性上调，VCAM-1和E-选择素的表达无明显变化。辐射诱导的HUVEC ICAM-1表达增加与中性粒细胞对照射内皮细胞的黏附增强有关。IL-6或辐射释放的其他可溶性因子不参与辐射后ICAM-1的表达增强。Northern印迹分析显示，ICAM-1mRNA在10Gy射线照射后1~6d呈高表达。我们的数据提示ICAM-1参与了辐射诱导的内皮细胞炎症反应。
Adhesion molecules play a key role in cellular traffic through vascular endothelium, in particular during the inflammatory response when leukocytes migrate from blood into tissues. Since inflammation is one of the major consequences of radiation injury, we investigated the effect of ionizing radiation on cell-surface expression of the intercellular adhesion molecule-1 (ICAM-1), the vascular cell adhesion molecule-1 (VCAM-1) and E-selectin in cultured human umbilical Vein endothelial cells (HUVEC). Flow cytometry performed on irradiated HUVEC revealed both a time- (from 2 to 10 days) and dose- (from 2 to 10 Gy) dependent up-regulation of basal expression of ICAM-1, and no induction of VCAM-1 or E-selectin. The radiation-induced increase in ICAM-1 expression on HUVEC was correlated with augmented adhesion of neutrophils on irradiated endothelial cells. Interleukin-6 (IL-6) or other soluble factors released by irradiation were not involved in the enhanced ICAM-1 expression by irradiation. Northern blot analysis showed an overexpression of ICAM-1 mRNA from 1 to 6 days after a 10 Gy exposure. Our data suggest that ICAM-1 participates in the radiation-induced inflammatory reaction of the endothelium.