Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina
Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina
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DOI：
10.1016/j.algal.2016.01.005
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发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Ali, Sohail T.
中科院分区：
文献类型：
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作者：
Beacham, Tracey A.;Ali, Sohail T.
在这里，我们报告的重组表达和活性的酿酒酵母2型二酰基甘油酰基转移酶DGA1的功能与本地微拟球藻基因平行。DGAl的表达改变了所产生的脂肪酸的链长分布，并反映了油酰辅酶A底物偏好。对总FAME含量的影响是中等的，并且最多升高38%。DGAl转基因的表达在整个培养生命周期中变化，并且观察到转基因的生长依赖性环境沉默的证据。据我们所知，这是转基因微生物中沉默和随后重置的第一个例子。这项研究的结果为藻类基因工程的功效以及将这些微生物用作化学制造的生物平台提供了有价值的见解。(C)© 2016 Elsevier B.V.版权所有。
Here we report recombinant expression and activity of the Saccharomyces cerevisiae type 2 diacylglycerol acyltransferase DGA1 functioning in parallel with the native Nannochloropsis salina genes. Expression of DGA1 shifted the chain length distribution of fatty acids produced and reflected an oleoyl-CoA substrate preference. Effect on the total FAME content was moderate and elevated by a maximum of 38%. Expression of the DGA1 transgene varied throughout the culture life cycle and evidence of growth dependent environmental silencing of the transgene was observed. This is to our knowledge the first example of silencing and subsequent resetting in a transgenic microalga. Results from this study add valuable insights into the efficacy of algal genetic engineering and use of these microorganisms as bio-platforms for chemical manufacture. (C) 2016 Elsevier B.V. All rights reserved.