The cloning of cyclin B3 and its gene expression during hormonally induced spermatogenesis in the teleost, Anguilla japonica
The cloning of cyclin B3 and its gene expression during hormonally induced spermatogenesis in the teleost, Anguilla japonica
复制标题
DOI：
10.1016/j.bbrc.2004.08.097
复制
发表时间：
2004-10-08
影响因子：
3.1
通讯作者：
Nagahama, Y
中科院分区：
文献类型：
--
作者：
Kajiura-Kobayashi, H;Kobayashi, T;Nagahama, Y
我们从鳗鱼睾丸中克隆了细胞周期蛋白B1、B2和B3的cDNA。北方印迹分析表明，这些细胞周期蛋白B mRNA的表达和增加，从第3天起的激素诱导的精子发生，细胞周期蛋白B3的表达最占主导地位的精子发生。原位杂交结果表明，cyclin B1和B2在精原细胞期至精母细胞期均有表达。另一方面，cyclin B3 mRNA仅在精原细胞中表达。虽然小鼠细胞周期蛋白B3特异性地表达于减数分裂前期早期，但这些结果表明，鳗鱼细胞周期蛋白B3在精子发生过程中仅限于精原细胞表达，而不存在于精母细胞中。这些事实共同表明，鳗鱼细胞周期蛋白B3是专门参与精原细胞增殖（有丝分裂），但不是在减数分裂。(C)2004爱思唯尔公司All rights reserved.
We cloned cyclin B1, B2, and B3 cDNAs from the eel testis. Northern blot analysis indicated that these cyclin B mRNAs were expressed and increased from day 3 onward after the hormonal induction of spermatogenesis, and that cyclin B3 was most dominantly expressed during spermatogenesis. In situ hybridization showed that cyclin B1 and B2 were present from the spermatogonium stage to the spermatocyte stage. On the other hand, cyclin B3 mRNA was present only in spermatogonia. Although mouse cyclin B3 is expressed specifically in the early meiotic prophase, these results indicate that eel cyclin B3 expression is limited during spermatogenesis to spermatogonia, but is not present in spermatocytes. These facts together suggest that eel cyclin B3 is specifically involved in spermatogonial proliferation (mitosis), but not in meiosis. (C) 2004 Elsevier Inc. All rights reserved.