Carboxymethyl- and carboxyl-catechins from ripe Pu-er tea.
Carboxymethyl- and carboxyl-catechins from ripe Pu-er tea.
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DOI：
10.1021/jf5036959
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发表时间：
2014-12
影响因子：
6.1
通讯作者：
Li-Wen Tian;Mu-Ke Tao;Min Xu;Jing Hu;Hong-Tao Zhu;Wen‑Yong Xiong;Dong Wang;Chong-ren Yang;Ying-Jun Zhang
中科院分区：
文献类型：
--
作者：
Li-Wen Tian;Mu-Ke Tao;Min Xu;Jing Hu;Hong-Tao Zhu;Wen‑Yong Xiong;Dong Wang;Chong-ren Yang;Ying-Jun Zhang
普洱熟茶是云南特有的微生物后发酵茶，自古以来就是以茶树叶片为原料，经绿色普洱茶加工而成。assamica（Theaceae）。茶红素的化学研究从商品普洱茶中分离出4个新的黄烷-3-醇衍生物，8-羧甲基-（+）-儿茶素（1），8-羧甲基-（+）-儿茶素甲酯（2），6-羧甲基-（+）-儿茶素（3）和6-羧甲基-（-）-没食子儿茶素（4），以及18个已知化合物，包括其它三种黄烷-3-醇衍生物（5-7）、10种黄酮苷（8-17）、两种可水解单宁（18和19）、两种奎尼酸衍生物（20-21）和嘌呤生物碱（22）。黄酮苷8-11为首次从茶树中分离得到。采用高效液相色谱法对普洱熟茶茶红素进行了定性分析，结果表明茶红素的主要成分为没食子酸。在分化的3 T3-L1脂肪细胞中测试化合物4、6-17、21和22对胰岛素敏感性的急性活性，但在10 μM的浓度下，它们都没有显示出显著的生物活性。
Ripe Pu-er tea, a special microbial postfermented tea originated from Yunnan Province, China, since ancient times, is made from green Pu-er tea prepared from the leaves of Camellia sinensis var. assamica (Theaceae). Chemical investigation on thearubigin (n-BuOH-soluble) fraction of the commercial ripe Pu-er tea, led to the identification of four new flavan-3-ol derivatives, 8-carboxymethyl-(+)-catechin (1), 8-carboxymethyl-(+)-catechin methyl ester (2), 6-carboxymethyl-(+)-catechin (3), and 6-carboxyl-(-)-gallocatechin (4), together with 18 known compounds, including other three flavan-3-ol derivatives (5-7), 10 flavonoid glycosides (8-17), two hydrolyzable tannins (18 and 19), two quinic acid derivatives (20-21), and a purine alkaloid (22). Flavonoid glycosides 8-11 are reported from tea plants for the first time. The thearubigin fraction of ripe Pu-er tea was qualitatively analyzed by HPLC, and gallic acid was found to be the major component. Compounds 4, 6-17, 21 and 22 were tested for their acute activities on insulin sensitivity in differentiated 3T3-L1 adipocytes, but none of them showed significant bioactivity at a concentration of 10 μM.