Longitudinal organization of the anterior neural plate and neural tube.
Longitudinal organization of the anterior neural plate and neural tube.
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发表时间：
1995-12
期刊：
影响因子：
4.6
通讯作者：
K. Shimamura;Dennis J. Hartigan;Salvador Martínez;L. Puelles;J. Rubenstein
中科院分区：
文献类型：
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作者：
K. Shimamura;Dennis J. Hartigan;Salvador Martínez;L. Puelles;J. Rubenstein
在上个世纪，已经提出了几种前脑组织的形态学模型，假设了组织原基关系的替代拓扑解决方案。所有这些模型的核心是它们对纵轴以及神经板和神经管的纵向组织的定义。为了了解前脑的纵向组织，我们试图识别沿着胚胎中枢神经系统整个纵轴连续的分子特性。在本文中，我们描述了对小鼠交配后 7.5 天（前体阶段）至 10.5 天 (dpc) 期间多个基因表达的研究，这些研究为前后轴的轨迹和前 CNS 的纵向组织提供了证据。具体来说，我们报告说，noggin、sonic hedgehog 和 Nkx-2.2 的表达定义了沿整个 CNS 轴存在的细胞纵向列。在前脑内，这些基因以及 Nkx-2.1 和 BF-1 的表达位于神经板和神经管的不同纵向区域。我们证明前脑最早的纵向轴突通路与 Nkx-2.2 定义的纵向域在空间上相关。最后，前一基因以及 Otx-1 和 Emx-2 的表达表明头侧神经板被组织成由纵向和横向边界界定的分子上不同的区域；这些结果为定义神经板模式机制提供了基础。
Over the last century, several morphological models of forebrain organization have been proposed that hypothesize alternative topological solutions for the relationships of the histogenic primordia. Central to all of these models are their definitions of the longitudinal axis and the longitudinal organization of the neural plate and neural tube. To understand the longitudinal organization of the anterior brain, we have sought to identify molecular properties that are continuous along the entire longitudinal axis of the embryonic CNS. In this essay, we describe studies of the expression of several genes in the mouse between 7.5 (presomite stage) and 10.5 days post coitum (dpc) that provide evidence for the trajectory of the anterior-posterior axis and the longitudinal organization of the anterior CNS. Specifically, we report that the expression of noggin, sonic hedgehog and Nkx-2.2 define longitudinal columns of cells that are present along the entire CNS axis. Within the forebrain, the expression of these genes, as well as that of Nkx-2.1 and BF-1, are in distinct longitudinal regions in the neural plate and tube. We demonstrate that the earliest longitudinal axon pathways of the forebrain are spatially correlated with the longitudinal domain defined by Nkx-2.2. Finally, expression of the former genes, and Otx-1 and Emx-2, suggests that the cephalic neural plate is organized into molecularly distinct domains delimited by longitudinal and transverse borders; these results provide a foundation for defining the mechanisms that pattern the neural plate.