a 1-Adrenoceptor Stimulation Enhances the Delayed Rectifier K ' Current of Guinea Pig Ventricular Cells Through the Activation of Protein Kinase C
a 1-Adrenoceptor Stimulation Enhances the Delayed Rectifier K ' Current of Guinea Pig Ventricular Cells Through the Activation of Protein Kinase C
复制标题
a 1-肾上腺素受体刺激通过激活蛋白激酶 C 增强豚鼠心室细胞的延迟整流 K 电流
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
M. Kanno
中科院分区：
文献类型：
--
作者：
N. Tohse;H. Nakaya;M. Kanno
使用膜片钳方法在分离的豚鼠心室细胞中检查钙肾上腺素受体刺激对延迟整流 K' 电流 (IK) 的影响。 'K 是通过在含有 3 μM 硝苯地平的不含 Na+ 和 K' 的溶液中从 -30 mV 的保持电位的 3 秒去极化脉冲诱发的。苯肾上腺素 (30 µM) 在普萘洛尔 (1 ELM) 存在下会导致 'K 增加。在 5 个细胞中，去氧肾上腺素使 'K 的尾电流增加了 23±5%。去氧肾上腺素的这种作用被哌唑嗪（0.3，uM）（一种选择性α-受体阻滞剂）阻断。去氧肾上腺素对 IK 的电压和时间依赖性仅产生很小的影响。用 1-(5-异喹啉基磺酰基)-2-甲基哌嗪 (H-7, 10,uM) 预处理消除了去氧肾上腺素诱导的 IK 增加。此外，用最大有效浓度的 12-O-十四烷酰佛波醇 13-乙酸酯 (100 nM) 进行预处理消除了去氧肾上腺素诱导的 IK 增加。总之，α1-肾上腺素受体刺激增加了豚鼠心肌细胞中的IK。这种 cvl 肾上腺素受体介导的反应可能与蛋白激酶 C 的激活有关。IK 的增加可以解释在豚鼠细胞中刺激 ae 肾上腺素受体后观察到的动作电位持续时间的缩短。 (Circulation Research 1992;71:1441-1446) 关键词 * a,-肾上腺素受体 * 延迟整流 K' 电流 * 蛋白激酶 C * 心肌细胞 去氧肾上腺素 * H-7 * 膜片钳
The effect of cal-adrenoceptor stimulation on the delayed rectifier K' current (IK) was examined in isolated guinea pig ventricular cells by use of the patch-clamp method. 'K was evoked by a 3-second depolarizing pulse from a holding potential of -30 mV in a Na+and K'-free solution containing 3 ,uM nifedipine. Phenylephrine (30 ,uM) in the presence of propranolol (1 ELM) produced an increase in 'K. In five cells, phenylephrine increased the tail current of 'K by 23±5%. This effect of phenylephrine was blocked by prazosin (0.3 ,uM), a selective al-blocker. Phenylephrine produced only a small effect on the voltage and time dependence of IK. Pretreatment with 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7, 10 ,uM) abolished the phenylephrine-induced increase in IK. In addition, pretreatment with a maximally effective concentration of 12-O-tetradecanoylphorbol 13-acetate (100 nM) abolished the phenylephrine-induced increase in IK. In conclusion, a!,-adrenoceptor stimulation increases IK in guinea pig cardiomyocytes. This cvl-adrenoceptor-mediated response may be related to an activation of protein kinase C. The increase in IK may explain a shortening of action potential duration observed after ae-adrenoceptor stimulation in guinea pig cells. (Circulation Research 1992;71:1441-1446) KEYWoRDs * a,-adrenoceptors * delayed rectifier K' current * protein kinase C * cardiomyocytes phenylephrine * H-7 * patch clamp
肌醇三磷酸可增强带皮心肌和骨骼肌中钙的释放。
DOI：
10.1152/ajpcell.1986.250.5.c807
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
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作者：
Nosek,TM;Williams,MF;Zeigler,ST;Godt,RE
通讯作者：
Godt,RE