Inflammatory profile dysregulation in nuclear workers occupationally exposed to low-dose gamma radiation
Inflammatory profile dysregulation in nuclear workers occupationally exposed to low-dose gamma radiation
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DOI：
10.1093/jrr/rrz059
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发表时间：
2019-11-01
影响因子：
2
通讯作者：
Boteva, Rayna
中科院分区：
文献类型：
--
作者：
Aneva, Nevena;Zaharieva, Elena;Boteva, Rayna
慢性炎症是一个共同点，它与各种健康状况有关，包括组织对辐射的反应。这项初步研究调查了炎性细胞因子--白细胞介素IL-6、IL-8、IL-10、单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子α（TNF α）--是否可用作职业受照者辐射引起的不良健康影响的早期生物标志物。该研究包括33名从核工业外部暴露于伽马辐射的工人，累积剂量为0.11至190 mSv，以及42名年龄和社会经济地位相当的非暴露对照。通过酶联试验（ELISA）分析血浆样品中的IL-6、IL-8、MCP-1、TNF α和IL-10。用比色法测定血浆总抗氧化状态（TAS）。辐射暴露组和对照组测量到MCP-1、TNF α和IL-10的显著不同水平。75%的放射工作者有高MCP-1水平或低IL-10水平，30%的人有三种细胞因子失调。大约50%的工人表现出上调的抗氧化状态，这似乎补偿了这些个体中促炎细胞因子的变化。相比之下，只有2%的对照受试者被发现有三个失调的细胞因子，所有这些都在正常的TAS范围内测量。目前的研究可能是朝着建立一套可靠的生物标志物，用于估计低辐射剂量人群的健康风险迈出的重要一步。
Chronic inflammation is a common denominator linking a wide range of health conditions, including tissue response to radiation exposure. This pilot study investigates whether inflammatory cytokines-interleukins IL-6, -8, -10, monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor alpha (TNF alpha)-can be used as early biomarkers of radiation-induced adverse health effects in occupationally exposed individuals. The study included 33 workers externally exposed to gamma radiation from the nuclear industry with cumulated doses from 0.11 to 190 mSv and 42 non-exposed controls of comparable age and socio-economic status. IL-6, IL-8, MCP-1, TNF alpha and IL-10 were analyzed by enzyme-linked assay (ELISA) in blood plasma samples. Total antioxidant status (TAS) of blood plasma was determined by a colorimetric assay. The radiation-exposed and control groups measured significantly different levels of MCP-1, TNF alpha and IL-10. Seventy-five percent of radiation workers had either high MCP-1 levels or low IL-10 levels and 30% had all three cytokines dysregulated. Approximately 50% of workers showed upregulated antioxidant status, which appeared to compensate the pro-inflammatory cytokine shift in these individuals. In contrast, only 2% of the control subjects were found to have three dysregulated cytokines, and all of them measured within the normal TAS range. The present study may represent an important step towards the establishment of a reliable set of biomarkers for health-risk estimation in population cohorts exposed to low radiation doses.