SYNCHRONIZED ERYTHROCYTIC SCHIZOGONY AND GAMETOCYTOGENESIS OF PLASMODIUM-BERGHEI INVIVO AND INVITRO
SYNCHRONIZED ERYTHROCYTIC SCHIZOGONY AND GAMETOCYTOGENESIS OF PLASMODIUM-BERGHEI INVIVO AND INVITRO
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DOI：
10.1017/s0031182000062673
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发表时间：
1985-01-01
期刊：
影响因子：
2.4
通讯作者：
VANDERKAAY, HJ
中科院分区：
文献类型：
--
作者：
MONS, B;JANSE, CJ;VANDERKAAY, HJ
伯氏疟原虫的无性和有性发育是同步的，没有化学干预，使用体外培养技术。结合体内外实验研究了配子体成熟度与年龄、形态的关系。裂殖期为22-23 h。在侵袭后26 h（p.i.）第一批雄性开始有了吐鞭毛虫的能力至20 h p.i.在Giemsa染色的涂片中可识别第一配子母细胞，但直到成熟（感染后26小时）才能区分性别。配子体的存活时间估计为26小时在体外（半衰期13小时），并建议同样的存活时间在体内配子体。伯氏疟原虫裂殖体在成熟后明显从外周循环中消失，几乎立即破裂。在非搅拌培养物中，体外成熟的细胞在腹膜内持续长达48小时。没有收集到任何配子母细胞亚群被隔离的证据。
Both asexual and sexual development of Plasmodium berghei was synchronized without chemical intervention using in vitro culture techniques. Combined in vivo and in vitro experiments were performed on the relationship between age, morphology and maturity of gametocytes. Schizogony took 22-23 h in the experiments. At 26 h post-invasion (p.i.) the first males became capable of exflagellation. By 20 h p.i. the first gametocytes were recognizable in Giemsa-stained smears but the sex was hardly distinguishable until maturity (26 h p.i.). Survival time of gametocytes was estimated at 26 h in vitro (half-life 13 h) and the same survival time was suggested for gametocytes in vivo. Schizonts of P. berghei apparently disappeared from the peripheral circulation upon maturity, rupturing almost immediately. Mature schizonts in vitro persisted up to 48 h i.p. in non-agitated cultures. No evidence was collected for sequestration of any sub-population of gametocytes.