Pathway choice in DNA double strand break repair: observations of a balancing act.
Pathway choice in DNA double strand break repair: observations of a balancing act.
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DOI：
10.1186/2041-9414-3-9
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发表时间：
2012-11-27
期刊：
影响因子：
--
通讯作者：
Gent DC
中科院分区：
文献类型：
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作者：
Brandsma I;Gent DC
DNA双链断裂（DSB）的正确修复对于保持基因组的完整性至关重要。DSB的修复主要有两种途径，同源重组（HR）和非同源末端连接（NHEJ）。由于需要姐妹染色单体作为模板，HR仅限于细胞周期的S期和G2期，而NHEJ在整个细胞周期中都有活性，并且不依赖于模板。两种途径之间的平衡对于基因组稳定性至关重要，并且已经开发了许多测定来测量两种途径的效率。已知几种蛋白质会影响HR和NHEJ之间的平衡，断裂的复杂性也起着作用。在这篇综述中，我们描述了几种修复检测，以确定这两种途径的效率。我们讨论了HR和NHEJ之间的平衡紊乱如何导致疾病，以及如何将其用于癌症治疗。
Proper repair of DNA double strand breaks (DSBs) is vital for the preservation of genomic integrity. There are two main pathways that repair DSBs, Homologous recombination (HR) and Non-homologous end-joining (NHEJ). HR is restricted to the S and G2 phases of the cell cycle due to the requirement for the sister chromatid as a template, while NHEJ is active throughout the cell cycle and does not rely on a template. The balance between both pathways is essential for genome stability and numerous assays have been developed to measure the efficiency of the two pathways. Several proteins are known to affect the balance between HR and NHEJ and the complexity of the break also plays a role. In this review we describe several repair assays to determine the efficiencies of both pathways. We discuss how disturbance of the balance between HR and NHEJ can lead to disease, but also how it can be exploited for cancer treatment.