Preparative high speed gel permeation chromatography of proteins on Toyopearl HW55F.
Preparative high speed gel permeation chromatography of proteins on Toyopearl HW55F.
复制标题
在 Toyopearl HW55F 上对蛋白质进行制备型高速凝胶渗透色谱。
DOI：
10.1016/0006-291x(81)91263-8
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
J. D. Karkas
中科院分区：
文献类型：
--
作者：
J. Germershausen;J. D. Karkas
A method is described for the rapid separation of protein mixtures by high speed gel permeation chromatography using Toyopearl HW55F, a new semi-rigid hydrophilic polymer. Good resolution of protein mixtures according to molecular size can be achieved on this material with high flow rates and low column pressures. Molecular weight estimations in the range between 104and 106daltons can be performed within minutes. Large-scale enzyme purification (up to 1.6 gm of starting material with a 3.2 × 105 cm column) was achieved with 86–110% recovery of enzymatic activity. Data are presented on the optimum column length, flow rate, loading capacity and eluant ionic strength.
