Cellular differentiation in the kidneys of newborn mice studies with the electron microscope.
Cellular differentiation in the kidneys of newborn mice studies with the electron microscope.
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用电子显微镜研究的新生小鼠肾脏中的细胞分化。
DOI：
10.1083/jcb.3.3.349
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发表时间：
1957-05-25
期刊：
影响因子：
--
通讯作者：
CLARK SL Jr
中科院分区：
文献类型：
--
作者：
CLARK SL Jr
瑞士白化病小鼠的肾脏结构在出生后的前2周内发生了渐进性变化。细胞增殖形成新的肾单位，细胞通过获得专门的膜成分而分化，出生时存在的某些细胞学特征大量减少或消失。用光学显微镜和电子显微镜研究了皮层小管细胞的分化。出生时，肾小管部分分化和部分分化。在出生后的前2周，刷状缘通过顶端细胞边缘大量微绒毛的积累在近端小管中发育。近端和远端小管中的基底条纹是线粒体排列的结果，这似乎是相邻凹槽细胞进行性联锁的结果。线粒体的数量和大小增加，积累均匀的基质，并获得小的，非常致密的颗粒。集合管的基细胞膜形成紧密的褶皱，并出现含有许多小胞质小泡和微绒毛的深色细胞。出生时，肾细胞中有密集的不规则胞质内含物，推测为Palade，Palade细胞质颗粒丰富，近端小管细胞中有大的圆形体。出生后几天，Palade包涵体消失，Palade细胞质颗粒随着细胞分化而大量减少。近端小管中的大圆形体由无定形物质组成，并含有同心层状结构和线粒体。它们类似于通过给予蛋白质在成年大鼠和小鼠的近端小管中产生的细胞质液滴。幼年小鼠出生后第一周，大圆形体从近端小管中消失，但成年小鼠可发现同心板层结构。
The structure of the kidney of the Swiss albino mouse changes progressively during the first 2 weeks after birth. Cells proliferate to form new nephrons, cells differentiate by acquiring specialized membranous components, and certain cytological features which are present at birth diminish in abundance or disappear. The differentiation of the cells of the cortical tubules has been studied using the light and electron microscopes. The tubules are partially and variably differentiated at birth. During the first 2 weeks after birth the brush border develops in the proximal tubules by the accumulation of numerous microvilli on the apical cell margins. Basal striations develop in proximal and distal tubules as an alignment of mitochondria, the result of what appears to be progressive interlocking of adjacent fluted cells. The mitochondria increase in number and size, accumulate homogeneous matrix, and acquire small, very dense granules. The collecting ducts develop tight pleating of the basal cell membranes, and dark cells containing numerous small cytoplasmic vesicles and microvilli appear. At birth there are dense irregular cytoplasmic inclusions presumed to be lipide in renal cells, the cytoplasmic granules of Palade are abundant, and there are large round bodies in the cells of the proximal tubules. The lipide inclusions disappear a few days after birth, and the cytoplasmic granules of Palade diminish in abundance as the cells differentiate. The large round bodies in the proximal tubules consist of an amorphous material and contain concentrically lamellar structures and mitochondria. They resemble the cytoplasmic droplets produced in the proximal tubules of adult rats and mice by the administration of proteins. The large round bodies disappear from the proximal tubules of infant mice during the first week after birth, but the concentric lamellar structures may be found in adult mice.