Reinnervation of the rat musculocutaneous nerve stump after its direct reconnection with the C5 spinal cord segment by the nerve graft following avulsion of the ventral spinal roots:: a comparison of intrathecal administration of brain-derived neurotrophic factor and Cerebrolysin
Reinnervation of the rat musculocutaneous nerve stump after its direct reconnection with the C5 spinal cord segment by the nerve graft following avulsion of the ventral spinal roots:: a comparison of intrathecal administration of brain-derived neurotrophic factor and Cerebrolysin
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DOI：
10.1007/s00221-004-1969-z
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发表时间：
2004-12-01
影响因子：
2
通讯作者：
Stejskal, L
中科院分区：
文献类型：
--
作者：
Haninec, P;Dubovy, P;Stejskal, L
采用C5腹侧神经根撕脱伤实验模型，评价脑源性神经营养因子（BDNF）和脑溶素治疗对运动神经元维持和存活以及残端神经功能再生的影响。与对照组相比，BDNF处理减少了运动神经元的丢失和萎缩，并促进了肌皮神经远端残端轴突芽的再生。但髓鞘纤维轴突直径明显小于对照组。形态计量学结果与行为学测试的低分相关，与给药大鼠相似。脑溶血素治疗可有效保护运动神经元免于细胞死亡。此外，髓鞘轴突的形态特征优于给药组和BDNF组。梳毛测试的平均得分表明运动神经功能重建的效果优于BDNF给药组。在BDNF和脑溶素处理的大鼠中，大部分通过神经移植再生的运动神经元表达了胆碱乙酰转移酶免疫染色。结果表明，BDNF在预防根撕脱伤后运动神经元死亡和损伤运动神经功能恢复方面的作用比脑溶素更温和。
Experimental model based on the C5 ventral root avulsion was used to evaluate the efficacy of brain-derived neurotrophic factor (BDNF) and Cerebrolysin treatment on motor neuron maintenance and survival resulted in the functional reinnervation of the nerve stump. In contrast to vehicle, BDNF treatment reduced the loss and atrophy of motor neurons and enhanced the regrowth axon sprouts into the distal stump of musculocutaneous nerve. However, the axon diameter of the myelinated fibers was smaller than those of control rats. The morphometric results were related to a low score in behavioral test similar to vehicle-treated rats. Cerebrolysin treatment greatly protected the motor neurons against cell death. Moreover, morphometric features of myelinated axons were better than those of rats treated with vehicle or BDNF. The mean score of grooming test suggested better results of the functional motor reinnervation than after BDNF administration. The majority of rescued motor neurons regenerating their axons through nerve graft in both BDNF- and Cerebrolysin-treated rats expressed choline acetyltransferase immunostaining. The results demonstrate that BDNF has more modest effects in preventing the death of motor neurons and functional recovery of injured motor nerve after root avulsion than Cerebrolysin.