Androgen-regulated proteins of rat seminal vesicle secretion constitute a structurally related family present in the copulatory plug
Androgen-regulated proteins of rat seminal vesicle secretion constitute a structurally related family present in the copulatory plug
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大鼠精囊分泌的雄激素调节蛋白构成了交配塞中存在的结构相关的家族
DOI：
10.1016/0303-7207(86)90149-8
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发表时间：
1986
影响因子：
4.1
通讯作者：
S. J. Higgins
中科院分区：
文献类型：
--
作者：
S. Fawell;D. Pappin;C. McDonald;S. J. Higgins
利用聚丙烯酰胺凝胶电泳技术，高收率地纯化了大鼠精囊中所有主要的雄激素调节分泌蛋白。在这个过程中，第六种以前未记载的蛋白质被鉴定出来。所有蛋白质的氨基酸组成非常相似，非常不寻常，赖氨酸和精氨酸含量高，40-50%的残基由丝氨酸、甘氨酸和谷氨酸/谷氨酰胺组成。其中3个蛋白的n端氨基酸序列表明它们明显是相关基因的产物。至少有一种其他蛋白质在体内被n端阻断。针对每种蛋白质的特异性抗体已经产生，并提供了蛋白质之间结构相似性的证据。抗体也被用于大鼠交配栓的免疫荧光组织化学，首次表明精囊分泌的所有主要蛋白质都是这种生殖结构的组成部分。
All the major androgen-regulated secretory proteins of rat seminal vesicles have been purified in high yield from polyacrylamide gels using electroelution. In the process a sixth previously undocumented protein has been identified. Amino acid compositions of all the proteins are very similar and highly unusual, being high in lysine and arginine, and with 40–50% of the residues accounted for by serine, glycine and glutamate/glutamine. N-Terminal amino acid sequences for 3 of the proteins show that they are clearly the products of related genes. At least one of the other proteins is N-terminally blocked in vivo. Antibodies specific for each protein have been raised and provide evidence of structural similarity between the proteins. The antibodies were also used in immunofluorescence histochemistry with the rat copulatory plug, showing for the first time that all the major proteins of seminal vesicle secretion are components of this reproductive structure.