FcRγ promotes contact hypersensitivity to oxazolone without affecting the contact sensitisation process in B6 mice.
FcRγ promotes contact hypersensitivity to oxazolone without affecting the contact sensitisation process in B6 mice.
复制标题
FcRγ 促进 B6 小鼠对 oxasolone 的接触超敏反应，而不影响接触致敏过程。
DOI：
10.1111/exd.12622
复制
发表时间：
2015
影响因子：
3.6
通讯作者：
Ra C.
中科院分区：
文献类型：
--
作者：
Nunomura S;Ohtsubo-Yoshioka M;Okayama Y;Terui T;Ra C.
特异性接触性过敏原的致敏过程是诱发变应性接触性皮炎所不可或缺的。恶唑酮是一种充分表征的接触性过敏原。既往研究表明，携带FcRγ亚基的免疫细胞是恶唑酮诱导的接触性超敏反应所必需的，但FcRγ亚基在恶唑酮致敏过程中的生物学功能尚不清楚。在这项研究中，我们发现FcRγ缺乏降低了小鼠对恶唑酮的耳肿胀反应。然而，我们发现恶唑酮致敏的FcRγ−/−小鼠和恶唑酮致敏的野生型（WT）小鼠在其引流淋巴结（LN）中具有相当数量的CD 11 c + MHCIIhi树突状细胞（DC）。此外，来自FcRγ−/−和WT小鼠的恶唑酮致敏LN细胞在恶唑酮钥孔血蓝蛋白负载后显示出相当大的干扰素γ（IFNγ）、白细胞介素4（IL-4）和IL-17 A产生。与这些数据一致，恶唑酮致敏的FcRγ−/−和FcRγ+/+LN细胞对用半抗原激发的WT未处理小鼠产生接触超敏反应。我们的研究结果清楚地表明，在实验小鼠模型中，FcRγ亚基正向调节对恶唑酮的接触超敏反应，而不影响接触致敏过程。
The process of sensitisation by specific contact allergens is indispensable for the induction of allergic contact dermatitis. Oxazolone is a well‐characterised contact allergen. Previous studies suggested that immune cells bearing the FcRγsubunit are essential for oxazolone‐induced contact hypersensitivity, but the biological functions of the FcRγsubunit in the process of sensitisation to oxazolone remain unknown. In this study, we show that FcRγdeficiency decreases ear‐swelling responses to oxazolone in mice. However, we found that oxazolone‐sensitised FcRγ−/−mice and oxazolone‐sensitised wild‐type (WT) mice have comparable numbers of CD11c+MHCIIhidendritic cells (DCs) in their draining lymph nodes (LNs). In addition, oxazolone‐sensitised LN cells from both FcRγ−/−and WT mice showed considerable production of interferon‐gamma (IFNγ), interleukin‐4 (IL‐4) and IL‐17A upon oxazolone‐keyhole limpet haemocyanin loading. Consistent with these data, oxazolone‐sensitised FcRγ−/−and FcRγ+/+LN cells conferred contact hypersensitivity to WT naïve mice challenged with the hapten. Our findings clearly indicate that, in an experimental mouse model, the FcRγsubunit positively regulates contact hypersensitivity to oxazolone without affecting the contact sensitisation process.