Regulation of the formation and trafficking of vesicles from Golgi by PCH family proteins during chemotaxis.
Regulation of the formation and trafficking of vesicles from Golgi by PCH family proteins during chemotaxis.
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DOI：
10.1016/j.bbamcr.2009.04.012
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Chung CY
中科院分区：
文献类型：
--
作者：
Lee S;Han JW;Leeper L;Gruver JS;Chung CY
Previous study demonstrated that WASP localizes on vesicles during Dictyostelium chemotaxis and these vesicles appear to be preferentially distributed at the leading and trailing edge of migrating cells. In this study, we have examined the role of PCH family proteins, Nwk/Bzz1p-like protein (NLP) and Syndapin-like protein (SLP), in the regulation of the formation and trafficking of WASP-vesicles during chemotaxis. NLP and SLP appear to be functionally redundant and deletion of both nlp and slp genes cause the loss of polararized F-actin organization and significant defects in chemotaxis. WASP and NLP are colocalized on vesicles and interactions between two molecules via the SH3 domain of NLP/SLP and the proline-rich repeats of WASP are required for vesicle formation from Golgi. Microtubules are required for polarized trafficking of these vesicles as vesicles showing high directed mobility are absent in cells treated with nocodazole. Our results suggest that interaction of WASP with NLP/SLP is required for the formation and trafficking of vesicles from Golgi to the membrane, which might play a central role in the establishment of cell polarity during chemotaxis.
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影响因子：
4
作者：
Kessels, MM;Dong, JX;Qualmann, B
通讯作者：
Qualmann, B
影响因子：
4
作者：
Heath, Robert J. W.;Insall, Robert H.
通讯作者：
Insall, Robert H.
DOI：
10.1083/jcb.200403120
发表时间：
2004-06-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carreno S;Engqvist-Goldstein AE;Zhang CX;McDonald KL;Drubin DG
通讯作者：
Drubin DG
影响因子：
4.8
作者：
Kamioka, Y;Fukuhara, S;Mochizuki, N
通讯作者：
Mochizuki, N
影响因子：
9.2
作者：
Aspenstrom, P
通讯作者：
Aspenstrom, P