THERMAL DEGRADATION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE SYNTHESIZED ALLICIN AND ALLICIN INCORPORATED IN GEL
THERMAL DEGRADATION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE SYNTHESIZED ALLICIN AND ALLICIN INCORPORATED IN GEL
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DOI：
10.2298/hemind1000003i
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发表时间：
2010-03-01
影响因子：
0.9
通讯作者：
Stanojevic, Ljiljana P.
中科院分区：
文献类型：
--
作者：
Ilic, Dusica P.;Nikolic, Vesna D.;Stanojevic, Ljiljana P.
大蒜（A ilium sativum L.）药理活性的主要载体是有机硫化合物，其中最重要的是大蒜素，一种磺酸硫酯或烯丙基硫磺酸盐。本文采用紫外/可见、红外光谱和核磁共振等多种光谱方法对合成和纯化的大蒜素进行了鉴定。建立了HPLC法测定大蒜素含量的方法。采用红外光谱法对大蒜素的热降解过程进行了监测。阐述了以聚丙烯酸卡波波尔940为原料生产大蒜素凝胶的方法。用圆盘扩散法测定了纯大蒜素和大蒜素凝胶的抑菌活性。为了确定大蒜素的抗氧化活性，进行了DPPH测试，证明在低浓度（1 mg.cm(-3)）下，大蒜素具有较高的DPPH自由基清除能力（90%）。
The main carriers of the pharmacological activity of garlic (A ilium sativum L.) are organic sulfur compounds, the most important among them being allicin, a sulfenic acid thioester, or allylthiosulfonate. In this paper, the identification of synthesized and purified allicin was determined by using various spectroscopic methods (UV/Vis, FTIR and NMR). An HPLC method was developed for the detection and determination of the allicin content. The thermal degradation of allicin by using FTIR method was monitored. The method for the production of allicin gel based on Carbopol 940 (poly(acrylic acid)) was elaborated. The antimicrobial activity of pure allicin and allicin incurporated into gel by using a disk diffusion method was determined. In order to determine the antioxidant activity of allicin DPPH testing was done and it was proved that with low concentrations (1 mg.cm(-3)) a high DPPH radicals scavenging capacity (90%) was achieved.