Distribution of poly(β-hydroxybutyrate) and poly(ε-caprolactone)aerobic degrading microorganisms in different environments
Distribution of poly(β-hydroxybutyrate) and poly(ε-caprolactone)aerobic degrading microorganisms in different environments
复制标题
聚(β-羟基丁酸酯)和聚(ε-己内酯)好氧降解微生物在不同环境中的分布
DOI：
10.1007/bf01458031
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Y. Tokiwa
中科院分区：
文献类型：
--
作者：
H. Nishida;Y. Tokiwa
为评价自然环境对塑料的降解能力，采用平板计数法，在含有乳化聚羟基丁酸酯（PHB）和聚己内酯（PCL）的琼脂培养基上，测定了土壤样品中降解聚β-羟基丁酸酯（PHB）和聚己内酯（PCL）的好氧微生物数量及其占微生物总数的比例。通过计数在平板上形成透明区的菌落来确定降解微生物的数量。结果发现，PHB和PCL降解（解聚）微生物分布在许多种材料，包括垃圾渗滤液，堆肥，污水污泥，森林土壤，农田土壤，水稻土，杂草田土壤，路边沙，池塘沉积物。在总菌落数中，降解PHB和PCL的微生物的百分比分别为0.2- 11.4%和0.8- 11.0%。结果表明，许多种降解微生物存在于每个环境中，并构建了不同的生物降解能力的特定财团。
To assess the capacity of the natural environment for degrading plastics, the populations of poly(β-hydroxybutyrate)(PHB)-and poly(e-caprolactone)(PCL)-degrading aerobic microorganisms and their ratios to the total number of microorganisms in soil samples were estimated by the plate count method with agar medium containing emulsified PHB or PCL. The numbers of the degrading microorganisms were determined by counting colonies that formed clear zones on the plate. It was found that PHB- and PCL-degrading (depolymerizing) microorganisms are distributed over many kinds of material, including landfill leachate, compost, sewage sludge, forest soil, farm soil, paddy soil, weed field soil, roadside sand, and pond sediment. Of total colony counts, the percentages of PHB and PCL degrading microorganisms were 0.2–11.4 and 0.8–11.0%, respectively. The results suggest that many kinds of degrading microorganisms are present in each environment and that specific consortia differing in biodegradation capacity are constructed.