Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum
Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum
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PtDGAT2B 的鉴定和表征，PtDGAT2B 是硅藻三角褐指藻中 DGAT2 酰基辅酶 A：二酰基甘油酰基转移酶家族的酰基转移酶
DOI：
10.1016/j.febslet.2013.01.015
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发表时间：
2013-03-01
期刊：
影响因子：
3.5
通讯作者：
Jiang, Mulan
中科院分区：
文献类型：
--
作者：
Gong, Yangmin;Zhang, Junping;Jiang, Mulan
二酰甘油酰基转移酶（DGAT）在某些产油生物的三酰甘油（TAG）合成过程中起着关键作用。我们在此描述了在三角褐指藻中对一种2型DGAT（PtDGAT2B）的鉴定，该硅藻含有4种推定的2型酰基辅酶A：DGAT，它们彼此之间序列相似性很低。通过表达PtDGAT2B，酿酒酵母TAG缺陷型四突变体中的TAG合成和脂滴形成能够完全恢复。PtDGAT2B的上调先于TAG的积累。体内酶活性功能分析表明，PtDGAT2B的表达可增加酵母TAG中不饱和C - 16和C - 18脂肪酸的比例。（C）2013欧洲生物化学学会联合会。由爱思唯尔B.V.出版。保留所有权利。
Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body formation could be completely restored in a Saccharomyces cerevisiae TAG-deficient quadruple mutant by expressing PtDGAT2B. Up-regulation of PtDGAT2B precedes the accumulation of TAG. Functional analysis of enzyme activity in vivo demonstrated that expression of PtDGAT2B can increase the proportion of unsaturated C-16 and C-18 fatty acids in yeast TAG. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.