FOXO3 Knockdown Accelerates Development of Bovine Primordial Follicles
FOXO3 Knockdown Accelerates Development of Bovine Primordial Follicles
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DOI：
10.1262/jrd.11-013h
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发表时间：
2011-08-01
影响因子：
1.8
通讯作者：
Miyano, Takashi
中科院分区：
文献类型：
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作者：
Bao, Rong-Mei;Hayakawa, Kohei;Miyano, Takashi
本研究的目的是阐明FOXO 3参与激活牛原始卵泡。免疫组化结果显示，FOXO 3在原始卵泡和初级卵泡的所有卵母细胞中均有表达。用FOXO 3小干扰RNA（siRNA）处理后，FOXO 3降低。用FOXO 3 siRNA处理含有主要原始卵泡的卵巢组织，然后异种移植到严重联合免疫缺陷（SCID）小鼠。异种移植后两个月，一些原始卵泡发育到第二和第三阶段，并且这些发育卵泡的总百分比（第二和第三卵泡：18 +/- 7%）高于用对照siRNA处理的对照移植物（7 +/- 1%）。据认为，牛原始卵泡激活受FOXO 3依赖性机制调节，FOXO 3的敲低诱导原始卵泡从FOXO 3抑制中释放，启动其生长。
The objective of the present study was to elucidate the involvement of FOXO3 in the activation of bovine primordial follicles. In immunohistochemistry, FOXO3 was detected in all of the oocytes in primordial and primary follicles. The FOXO3 decreased after treatment with FOXO3 small interfering RNAs (siRNAs). Ovarian tissues containing dominantly primordial follicles were treated with FOXO3 siRNAs and then xenografted to severe combined immune deficiency (SCID) mice. Two months after xenografting, some primordial follicles developed to the secondary and tertiary stages, and the total percentage of these developing follicles (secondary and tertiary follicles: 18 +/- 7%) was higher than in the control grafts treated with control siRNA (7 +/- 1%). It is thought that bovine primordial follicle activation is regulated by the FOXO3-dependent mechanism and that knockdown of FOXO3 induces the release of primordial follicles from FOXO3 suppression, initiating their growth.