Reduced diversity and altered composition of the gut microbiome in individuals with myalgic encephalomyelitis/chronic fatigue syndrome.
Reduced diversity and altered composition of the gut microbiome in individuals with myalgic encephalomyelitis/chronic fatigue syndrome.
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DOI：
10.1186/s40168-016-0171-4
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发表时间：
2016-06-23
期刊：
影响因子：
15.5
通讯作者：
Hanson MR
中科院分区：
文献类型：
--
作者：
Giloteaux L;Goodrich JK;Walters WA;Levine SM;Ley RE;Hanson MR
胃肠道紊乱是肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）患者常报告的症状之一。然而，ME/CFS是否与微生物组改变有关仍不确定。在这里，我们通过对病例（n = 48）和对照组（n = 39）粪便中的16S核糖体核糖核酸（rRNA）基因以及血清中的炎症标志物进行测序，分析了肠道微生物多样性。我们还检测了血液中的一系列炎症标志物：c反应蛋白（CRP）、肠脂肪酸结合蛋白（I-FABP）、脂多糖（LPS）、脂多糖结合蛋白（LBP）和可溶性CD14 （sCD14）。我们观察到ME/CFS患者中一些微生物易位的血液标志物水平升高；在ME/CFS受试者中，LPS、LBP和sCD14水平升高。LBP水平与LPS和sCD14相关，LPS水平与sCD14相关。通过细菌rRNA标记物的深度测序，我们确定了健康个体和ME/CFS患者肠道微生物组之间的差异。我们观察到，与对照组相比，ME/CFS标本中的细菌多样性减少，特别是厚壁菌门成员的相对丰度和多样性减少。在患者队列中，我们发现较少的多样性以及通常被报道为促炎物种的特定物种的增加和通常被描述为抗炎物种的减少。使用从16S rRNA和炎症标记物获得的数据训练的机器学习方法，个体被正确分类为ME/CFS，交叉验证准确率为82.93%。我们的研究结果表明，这种疾病中肠道微生物群的生态失调，并进一步表明微生物易位的发生率增加，这可能在ME/CFS的炎症症状中起作用。本文的在线版本（doi:10.1186/s40168-016-0171-4）包含补充材料，可供授权用户使用。
Gastrointestinal disturbances are among symptoms commonly reported by individuals diagnosed with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). However, whether ME/CFS is associated with an altered microbiome has remained uncertain. Here, we profiled gut microbial diversity by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from stool as well as inflammatory markers from serum for cases (n = 48) and controls (n = 39). We also examined a set of inflammatory markers in blood: C-reactive protein (CRP), intestinal fatty acid-binding protein (I-FABP), lipopolysaccharide (LPS), LPS-binding protein (LBP), and soluble CD14 (sCD14). We observed elevated levels of some blood markers for microbial translocation in ME/CFS patients; levels of LPS, LBP, and sCD14 were elevated in ME/CFS subjects. Levels of LBP correlated with LPS and sCD14 and LPS levels correlated with sCD14. Through deep sequencing of bacterial rRNA markers, we identified differences between the gut microbiomes of healthy individuals and patients with ME/CFS. We observed that bacterial diversity was decreased in the ME/CFS specimens compared to controls, in particular, a reduction in the relative abundance and diversity of members belonging to the Firmicutes phylum. In the patient cohort, we find less diversity as well as increases in specific species often reported to be pro-inflammatory species and reduction in species frequently described as anti-inflammatory. Using a machine learning approach trained on the data obtained from 16S rRNA and inflammatory markers, individuals were classified correctly as ME/CFS with a cross-validation accuracy of 82.93 %. Our results indicate dysbiosis of the gut microbiota in this disease and further suggest an increased incidence of microbial translocation, which may play a role in inflammatory symptoms in ME/CFS. The online version of this article (doi:10.1186/s40168-016-0171-4) contains supplementary material, which is available to authorized users.