Arterial and microvascular supply of cerebral hemispheres in the nude mouse revealed using corrosion casting and scanning electron microscopy
Arterial and microvascular supply of cerebral hemispheres in the nude mouse revealed using corrosion casting and scanning electron microscopy
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DOI：
10.1111/joa.12791
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发表时间：
2018-05
影响因子：
2.4
通讯作者：
S. Sangiorgi;A. Benedictis;M. Reguzzoni;A. Trezza;S. Cossu;C. Marras;S. Bellocchi;A. Manelli;M. Protasoni
中科院分区：
文献类型：
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作者：
S. Sangiorgi;A. Benedictis;M. Reguzzoni;A. Trezza;S. Cossu;C. Marras;S. Bellocchi;A. Manelli;M. Protasoni
脑血管化的形态学分析不仅对于表征血管和神经组织之间的解剖和生理关系很重要，而且对于理解在影响脑的许多病理条件下发生的结构修饰也是必需的。本研究的目的是在裸鼠大脑中生成大脑半球的三维血管图，这是一种广泛用于研究肿瘤生物学的动物模型。我们采用腐蚀铸型（CC）技术从30只裸鼠脑中分离血管。使用扫描电子显微镜（SEM）分析所有管型，生成关于血管长度和直径以及血管间和分支间距离的定量数据。我们确定了三个不同的地形区域：（i）皮质区，其特征在于表面宽的血管片，产生穿透灰质的末端穿通血管;（ii）灰质的内部，其中致密的毛细血管网形成许多向灰白质边界延伸的片状结构，穿通血管最终改变方向并形成明确的血管片;和（iii）白色物质层，其特征在于更无序的血管结构。在这项研究中，我们证明了CC-SEM方法在揭示正常裸鼠脑血管网络的3D拓扑结构方面的准确性。这些基线数据将作为未来使用裸鼠模型对病理学改变（如肿瘤浸润）进行解剖学研究的参考。
Morphological analyses of cerebral vascularization are not only important for the characterization of the anatomical and physiological relationships between vascular and nervous tissue, but also required to understand structural modifications that occur in many pathological conditions affecting the brain. The aim of this study was to generate a three‐dimensional vascular map of the cerebral hemispheres in the nude mouse brain, a widely used animal model for studying tumour biology. We used the corrosion casting (CC) technique to isolate blood vessels from 30 nude mouse brains. All casts were analysed using scanning electron microscopy (SEM), which generated quantitative data regarding vessel length and diameter as well as inter‐vascular and inter‐branching distances. We identified three different topographical regions: (i) the cortical region, characterized by a superficial wide sheet of vessels giving rise to terminal perforant vessels that penetrate the grey matter; (ii) the inner part of the grey matter, in which dense capillary nets form many flake‐like structures extending towards the grey–white matter boundary, where perforant vessels finally change direction and form a well‐defined vascular sheet; and (iii) the white matter layer, characterized by a more disorganized vascular architecture. In this study, we demonstrate the accuracy of the CC‐SEM method in revealing the 3D‐topographical organization of the vascular network of the normal nude mouse brain. These baseline data will serve as a reference for future anatomical investigations of pathological alterations, such as tumour infiltrations, using the nude mouse model.