[Effect of Shensu Yin on the expression of toll-like receptors and the downstream signaling components on RAW 264.7 cells].
[Effect of Shensu Yin on the expression of toll-like receptors and the downstream signaling components on RAW 264.7 cells].
复制标题
参素饮对RAW 264.7细胞Toll样受体及其下游信号成分表达的影响
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Ting
中科院分区：
文献类型：
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作者：
Bao;Lan;Yue;Shu;Canghai Li;H. Huo;Ting
目的
观察参苏饮对RAW 264.7细胞TLR 3、TLR 4表达及下游因子的影响。7个细胞。
方法
分别用脂多糖和POLY I：C刺激RAW264.7细胞，同时用参苏饮含药血清处理。24 h后，收集上清液，测定炎症因子TNF-α和IFN-β，提取mRNA，测定下游TLR 3、TLR 4等相关指标的表达，分析评价参苏饮药效作用的物质基础。
结果
参苏饮含药血清可抑制TLR 4、MyD 88、TRAF-6、TRAM和TRIF mRNA的表达，从而降低TNF-α和IFN-β的含量。
结论
抑制TLR 3、MyD 88、TRAM和TRIF mRNA的表达可能是参苏饮发挥清热解毒作用的基础。
OBJECTIVE
To investigate the influences of Shensu Yin to RAW 264.7 on the expression of TLR3, TLR4 and the factors of the downstream in RAW 264. 7 cells.
METHOD
RAW 264.7 cell line was stimulated with Lipopolysaccharide and POLY I: C, respectively, and treated with the drug serum of Shensuyin simultaneously. 24 hours later, collected the supernatant and measured the inflammatory factors TNF-alpha and IFN-beta, extracted mRNA and measured the expression of TLR3, TLR4 and other correlated indexes of the downstream, analyzed and evaluated Shensu Yin's substance basis of pharmacodynamic actions.
RESULT
Shensu Yin drug serum depressed the expression of TLR4, MyD88, TRAF-6, TRAM and TRIF mRNA, as a result, it decreased the amount of TNF-alpha and IFN-beta.
CONCLUSION
Depressing the expression of TLR3, MyD88, TRAM and TRIF mRNA may be the elementary basis of Shensu Yin to play heat-clearing and detoxicating effect.