Effects of manual acupuncture combined with donepezil in a mouse model of Alzheimer's disease
Effects of manual acupuncture combined with donepezil in a mouse model of Alzheimer's disease
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手法针刺联合多奈哌齐对阿尔茨海默病小鼠模型的影响
DOI：
10.1136/acupmed-2016-011310
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发表时间：
2019-02-01
影响因子：
2.5
通讯作者：
Li, Zhigang
中科院分区：
文献类型：
--
作者：
Jiang, Jing;Liu, Gang;Li, Zhigang
目的：方法：将40只7.5月龄快速衰老小鼠（SAMP 8）随机分为4组：SAMP 8组、SAMP 8 + D组、SAMP 8 + MA组和SAMP 8 + D+ MA组。另外10只7.5月龄SAMR 1雄性小鼠作为健康对照组（SAMR 1）。SAMP 8 + D组小鼠给予0.65 μ g/g/天剂量的多奈哌齐; SAMP 8 + MA组小鼠每天在GV 20、GV 26和印堂进行20分钟的手刺; SAMP 8 + D+ MA组小鼠接受多奈哌齐和手刺; SAMR 1和SAMP 8组小鼠仅进行束缚以控制处理效果。给药15 d后，采用Morris水迷宫实验、micro-PET（正电子发射断层扫描），H& E采用苏木精-伊红染色和免疫组化方法研究多奈哌齐与（SAMP 8 + D），针灸（SAMP 8 + MA）和多奈哌齐联合针刺本发明提供了用于治疗阿尔茨海默病的（SAMP 8 + D+ MA）疗法。我们发现，与未经治疗的SAMP 8组相比，多奈哌齐，手针，多奈哌齐与手针联合治疗均能改善大鼠空间学习记忆能力、脑内葡萄糖代谢水平及皮质A ss淀粉样蛋白含量。此外，在SAMP 8小鼠中，联合治疗的效果优于单独使用多奈哌齐或针刺治疗。结论：本研究表明，在阿尔茨海默病动物模型中，手法针刺和多奈哌齐联合治疗上级单独使用针刺和多奈哌齐。然而，应进行随机对照试验，以阐明联合治疗的临床疗效。
Objectives: To explore whether combined therapy with donepezil and acupuncture is better than treatment with donepezil or acupuncture individually in a rat model of Alzheimer's disease.Methods: In this study, we randomly divided 40 7.5-month-old senescence-accelerated mouse prone 8 (SAMP8) male mice into four groups: SAMP8, SAMP8+ D, SAMP8+ MA and SAMP8+ D+ MA. An additional 10 7.5-month-old SAMR1 male mice were included as a healthy control group (SAMR1). Mice in the SAMP8+ D group were given donepezil at a dose of 0.65 mu g/g/day; mice in the SAMP8+ MA group underwent manual acupuncture at GV20, GV26 and Yintang for 20 min per day; mice in the SAMP8+ D+ MA received both donepezil and manual acupuncture; and mice in the SAMR1 and SAMP8 groups underwent restraint only to control for the effects of handling. After the 15-day treatment, the Morris water maze test, micro-PET(positron-emission tomography), H& E (haematoxylin and eosin) staining, and immunohistochemistry were used to study the differences between donepezil (SAMP8+ D), acupuncture (SAMP8+ MA), and donepezil combined with acupuncture (SAMP8+ D+ MA) therapy for the treatment of Alzheimer's disease.Results: We found that, compared with the untreated SAMP8 group, donepezil, manual acupuncture, and combined therapy with donepezil and manual acupuncture all improved spatial learning and memory ability, the level of glucose metabolism in the brain, and the content of A ss amyloid in the cortex. Moreover, combined therapy outperformed treatment with donepezil or acupuncture individually in the SAMP8 mice.Conclusion: This study shows that the combination of manual acupuncture and donepezil in an Alzheimer's disease animal model is superior to acupuncture and donezepil alone. However, randomised controlled trials should be undertaken to clarify the clinical efficacy of combination therapy.