Structural organization of developing acetylcholine receptor aggregates
Structural organization of developing acetylcholine receptor aggregates
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DOI：
10.1002/(sici)1097-4695(19970605)32:6
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发表时间：
1997-06-05
期刊：
影响因子：
--
通讯作者：
Stollberg, J
中科院分区：
文献类型：
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作者：
Kunkel, DD;Stollberg, J
我们报告的第一个定量超微结构分析新形成的乙酰胆碱受体聚集体。在非洲爪蟾肌细胞培养物中诱导聚集体，用金颗粒标记，并使用高分辨率扫描电子显微镜检测。聚集体在聚集蛋白刺激后2小时内在超微结构水平上很容易辨别。发展中的聚集体的尺寸和密度分布表明，受体在小的“纳米聚集体”中非常迅速地达到最大密度，并且聚集过程不受受体扩散速率的限制。标记位置的定量分析表明，聚集体内的受体分布是非随机的。相反，新聚集的受体似乎被绑定到一个本地化的支架符合一个六边形（紧密堆积）的几何形状，间距约为9.9 nm。(C)John Wiley & Sons，Inc.
We report the first quantitative ultrastructural analysis of newly formed acetylcholine receptor aggregates. Aggregates were induced in Xenopus muscle cell cultures with agrin, labeled with gold particles, and detected using high resolution scanning electron microscopy. Aggregates are readily discernible at the ultrastructural level within 2 h of stimulation by agrin. The size and density profiles of the developing aggregates show that receptors reach maximal density very quickly in small ''nano-aggregates'' and that the aggregation process is not limited by the diffusion rate of the receptor. Quantitative analysis of label locations indicates that the receptor distribution within aggregates is nonrandom. Instead, the newly aggregated receptors appear to be bound to a localized scaffold conforming to a hexagonal (close-packed) geometry with a spacing of approximately 9.9 nm. (C) 1997 John Wiley & Sons, Inc.