S100A8/A9 Induces Apoptosis and Inhibits Metastasis of CasKi Human Cervical Cancer Cells
S100A8/A9 Induces Apoptosis and Inhibits Metastasis of CasKi Human Cervical Cancer Cells
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DOI：
10.1007/s12253-009-9225-2
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发表时间：
2010-09-01
影响因子：
2.8
通讯作者：
Geng, Li
中科院分区：
文献类型：
--
作者：
Qin, Fengjin;Song, Yao;Geng, Li
S100蛋白是一个Ca2+结合蛋白家族，近年来与几种人类疾病有关。据报道，S100蛋白（包括S100A9及其伴侣S100A8）的失调控表达与肿瘤疾病有关。在我们之前的研究中，通过对基因表达的系列分析，我们发现S100A9和S100A8在人宫颈鳞状细胞癌中表达减少。为了研究S100A8和S100A9在宫颈癌中的作用，我们纯化了重组S100A8和S100A9蛋白，并用这些蛋白治疗CaSki人宫颈癌细胞。结果发现，S100A8/A9可诱导CaSki细胞凋亡，抑制CaSki细胞迁移；S100A8/A9还能降低CaSki细胞中基质金属蛋白酶(MMP)-2的表达。综上所述，本研究提示S100A8和S100A9通过诱导细胞凋亡对CaSki癌细胞的增殖有抑制作用，并对CaSki细胞的侵袭性有抑制作用。
S100 proteins, a family of Ca2+-binding proteins, have been linked to several human diseases in recent years. Deregulated expression of S100 proteins, including S100A9 and its partner S100A8, was reported to be associated with neoplastic disorders. In our previous study using serial analysis of gene expression, we identified decreased expressions of S100A9 and S100A8 in human cervical squamous cell carcinoma. To investigate the functions of S100A8 and S100A9 in cervical cancer, we purified recombinant S100A8 and S100A9 proteins and treated CaSki human cervical cancer cells with these proteins. We found that S100A8/A9 induced apoptosis and inhibited migration of CaSki cells; S100A8/A9 also reduced the expression of matrix metalloproteinase (MMP)-2 in CaSki cells. In summary, this study suggests that S100A8 and S100A9 have inhibitory effects on the proliferation of CaSki carcinoma cells by inducing cell apoptosis and on the invasiveness of CaSki cells.