In vivo cardiac electrophysiology studies in the mouse
In vivo cardiac electrophysiology studies in the mouse
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DOI：
10.1161/01.cir.94.10.2641
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发表时间：
1996-11-15
期刊：
影响因子：
37.8
通讯作者：
Mendelsohn, ME
中科院分区：
文献类型：
--
作者：
Berul, CI;Aronovitz, MJ;Mendelsohn, ME
背景本报告描述了一种新的在体小鼠心外膜心脏电生理学研究的基础上，用于评估人类患者的心脏传导的临床协议。该技术允许广泛的电生理评价，包括对起搏，程控刺激，和pharmacological agents.Methods和结果的反应表面六导联心电图数据从18 C57 BL/6 J小鼠。总结了18只小鼠中14只的正常心脏传导特性，包括窦房结恢复时间、AV传导特性以及心房、AV和心室有效不应期的测定。在施用普鲁卡因胺（n=3）或奎尼丁（n=3）后，研究了六只小鼠的亚组。普鲁卡因胺组中的所有动物自发地发展为二度或完全AV阻滞。普鲁卡因胺或奎尼丁的窦性周期长度和不应期延长，但没有快速心律失常可以诱导心房或心室程序stimulation.Conclusions小鼠电生理方法可以快速评估小鼠心脏的传导特性。分析正常和转基因小鼠心脏传导的能力为检查正常生理和疾病状态下的分子电生理机制提供了有力的工具。
Background This report describes a novel in vivo mouse epicardial cardiac electrophysiology study based on clinical protocols used to evaluate cardiac conduction in human patients. The technique allows extensive electrophysiological evaluation, including the response to pacing, programmed stimulation, and pharmacological agents.Methods and Results Surface six-lead ECG data from 18 C57BL/6J mice are presented. Normal cardiac conduction properties for 14 of 18 mice that underwent the procedure are summarized, including determination of sinus node recovery times, AV conduction properties, and atrial, AV, and ventricular effective refractory periods. A subset of six mice was studied after the administration of either procainamide (n=3) or quinidine (n=3). All animals in the procainamide group developed either second-degree or complete AV block spontaneously. The sinus cycle length and refractory periods prolonged on procainamide or quinidine, but no tachyarrhythmias could be induced with atrial or ventricular programmed stimulation.Conclusions This mouse electrophysiology method allows rapid assessment of the conduction properties of the murine heart. The ability to analyze cardiac conduction in normal and transgenic mice provides a powerful tool for examining molecular electrophysiological mechanisms in normal physiology and disease states.