Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein cattle.
Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein cattle.
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DOI：
10.7150/ijbs.7.1016
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发表时间：
2011
影响因子：
9.2
通讯作者：
Zhong J
中科院分区：
文献类型：
--
作者：
Huang J;Ju Z;Li Q;Hou Q;Wang C;Li J;Li R;Wang L;Sun T;Hang S;Gao Y;Hou M;Zhong J
microRNA介导的转录后基因调控在包括牛在内的哺乳动物雌雄生殖器官和生殖细胞的发育和功能中起着重要作用。本研究通过Solexa测序技术与生物信息学相结合的方法，在荷斯坦牛睾丸和卵巢中发现了新的差异表达的miRNAs。在睾丸和卵巢组织中共鉴定出100和104种新的pre-miRNAs，分别编码122和136种成熟miRNAs。其中，6个miRNAs似乎是牛特异性的。共有246种已知的miRNAs在睾丸和卵巢组织中共表达。在已知的miRNAs中，发现了21个睾丸特异性miRNAs和9个卵巢特异性miRNAs（1-23 reads）。在本研究中发现大约30.5%的已知牛miRNAs在两个相应的生殖器官系统中具有>2倍的差异表达。推测的miRNA靶基因参与生殖生理相关的途径。通过实时定量PCR分析已知和新的组织特异性miRNA在奶牛中的表达。这项研究扩大了已知在牛中表达的miRNAs的数量。牛睾丸和卵巢组织中miRNA表达模式存在显著差异，这为研究miRNA表达的性别差异提供了重要信息。不同的miRNAs可能在荷斯坦牛生殖器官的发育过程中发挥重要的调控作用。
The posttranscriptional gene regulation mediated by microRNA plays an important role in the development and function of male and female reproductive organs and germ cells in mammals, including cattle. In the present study, we identified novel and differentially expressed miRNAs in the testis and ovary in Holstein cattle by combining the Solexa sequencing with bioinformatics. In total 100 and 104 novel pre-miRNAs were identified in testicular and ovarian tissues, encoding 122 and 136 mature miRNAs, respectively. Of these, 6 miRNAs appear to be bovine-specific. A total of 246 known miRNAs were co-expressed in the testicular and ovarian tissues. Of the known miRNAs, twenty-one testis-specific and nine ovary-specific (1-23 reads) were found. Approximately 30.5% of the known bovine miRNAs in this study were found to have >2-fold differential expression within the two respective reproductive organ systems. The putative miRNA target genes of miRNAs were involved in pathways associated with reproductive physiology. Both known and novel tissue-specific miRNAs are expressed by Real-time quantitative PCR analysis in dairy cattle. This study expands the number of miRNAs known to be expressed in cattle. The patterns of miRNAs expression differed significantly between the bovine testicular and ovarian tissues, which provide important information on sex differences in miRNA expression. Diverse miRNAs may play an important regulatory role in the development of the reproductive organs in Holstein cattle.
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影响因子：
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作者：
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