Metabolism and toxicological detection of the designer drug 4-chloro-2,5-dimethoxyamphetamine in rat urine using gas chromatography-mass spectrometry
Metabolism and toxicological detection of the designer drug 4-chloro-2,5-dimethoxyamphetamine in rat urine using gas chromatography-mass spectrometry
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DOI：
10.1007/s00216-008-1917-z
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发表时间：
2008-04-01
影响因子：
4.3
通讯作者：
Maurer, Hans H.
中科院分区：
文献类型：
--
作者：
Ewald, Andreas H.;Ehlers, Dorothea;Maurer, Hans H.
研究苯丙胺衍生的设计师药物4-氯-2，5-二甲氧基苯丙胺（DOC）在大鼠尿液中使用气相色谱-质谱技术的代谢和毒理学分析。确定的代谢产物表明，DOC代谢的O-去甲基化的苯环的位置2或5部分，其次是葡萄糖醛酸化和/或硫酸化。作者在酸水解、液-液萃取和微波辅助乙酰化后使用全扫描气相色谱-质谱法的系统毒理学分析程序允许检测大鼠尿液中对应于普通吸毒者剂量的DOC剂量的摄入。假设代谢相似，所述STA程序应适合作为人体尿液中DOC摄入的证据。
Studies are described on the metabolism and the toxicological analysis of the amphetamine-derived designer drug 4-chloro-2,5-dimethoxyamphetamine (DOC) in rat urine using gas chromatographic-mass spectrometric techniques. The metabolites identified indicated that DOC was metabolized by O-demethylation at position 2 or 5 of the phenyl ring partly followed by glucuronidation and/or sulfation. The authors' systematic toxicological analysis procedure using full-scan gas chromatography-mass spectrometry after acid hydrolysis, liquid-liquid extraction and microwave-assisted acetylation allowed the detection of an intake of a dose of DOC in rat urine that corresponds to a common drug user's dose. Assuming similar metabolism, the STA procedure described should be suitable as proof of an intake of DOC in human urine.