Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation
Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation
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DOI：
10.1016/j.bmcl.2004.03.023
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发表时间：
2004-05-17
影响因子：
2.7
通讯作者：
Nagamune, T
中科院分区：
文献类型：
--
作者：
Takeda, S;Tsukiji, S;Nagamune, T
我们开发了一种合成均质 DNA-蛋白质缀合物的便捷方法。该方法基于内含肽融合蛋白和半胱氨酸衍生的寡核苷酸的表达蛋白连接。使用新试剂 I 合成了一系列半胱氨酰寡核苷酸，并成功应用于表达蛋白连接，将寡核苷酸特异性连接到重组蛋白的 C 末端。 (C) 2004 Elsevier Ltd. 保留所有权利。
We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent I and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein. (C) 2004 Elsevier Ltd. All rights reserved.