Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris
Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris
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DOI：
10.1016/j.procbio.2004.06.046
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Zhong, JJ
中科院分区：
文献类型：
--
作者：
Mao, XB;Eksriwong, T;Zhong, JJ
通过在摇瓶中深层培养中药材蛹虫草，研究了不同碳源和碳/氮比对有用生物活性代谢物虫草素（3'-脱氧腺苷）生产的影响。所检查的碳源是乳糖、蔗糖、葡萄糖、果糖、半乳糖、麦芽糖和木糖，并且发现葡萄糖最有利于虫草素的产生，而细胞在半乳糖培养基中生长得最好。根据研究，细胞干重 (DW) 随着初始葡萄糖浓度在 25-70 g/l 范围内的增加而增加。在含有 40 g 葡萄糖/l 的培养基中获得了最高的虫草素产量，即第 18 天时的 245.7 +/- 4.4 mg/l。为了进一步提高虫草素产量，利用中心复合材料设计和响应面分析研究了碳/氮比例的影响。在具有优化碳源和氮源（即 42.0 g 葡萄糖/l 和 15.8 g 蛋白胨/l）的培养基中，虫草素的最大产量和生产率为每天 345.4 +/- 8.5 mg/l 和 19.2 +/- 0.5 mg/l。所得信息有助于大规模地下培养蛹虫草超产虫草素。 (c) 2004 Elsevier Ltd. 保留所有权利。
Effects of various carbon sources and carbon/nitrogen ratios on production of a useful bioactive metabolite, cordycepin (3'-deoxyadenosine), by submerged cultivation of a Chinese traditional medicinal mushroom Cordyceps militaris were investigated in shake flasks. The carbon sources examined were lactose, sucrose, glucose, fructose, galactose, maltose and xylose, and glucose was found to be most favourable to cordycepin production, whereas cells grew best in galactose medium. The dry cell weight (DW) was increased with an increase in initial glucose concentration within the range of 25-70 g/l as investigated. The highest cordycepin production, i.e. 245.7 +/- 4.4 mg/l on day 18, was obtained in medium containing 40 g glucose/l. To enhance further the cordycepin production, the effect of carbon/nitrogen ratios was studied using central composite design and response surface analysis. The maximum cordycepin production and productivity of 345.4 +/- 8.5 mg/l and 19.2 +/- 0.5 mg/l per day were achieved in medium with optimized carbon and nitrogen sources, i.e. 42.0 g glucose/l and 15.8 g peptone/l. The information obtained is helpful for the hyperproduction of cordycepin by submerged cultivation of C. militaris on a large scale. (c) 2004 Elsevier Ltd. All rights reserved.