Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers.
Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers.
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共表达红细胞和巨核细胞标记物的人白血病细胞系对血小板衍生生长因子类似物的组成型和诱导性分泌。
DOI：
10.1172/jci112895
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Ross,R
中科院分区：
文献类型：
--
作者：
Papayannopoulou,T;Raines,E;Collins,S;Nakamoto,B;Tweeddale,M;Ross,R
我们研究了多种人类造血细胞系中血小板衍生生长因子（PDGF）样蛋白的组成性和诱导性分泌。在四种人红白血病细胞系中注意到最高水平的分泌蛋白，除了红系谱系标记物之外，还表达一种或多种巨核细胞谱系标记物。诱导这些线的12-O-十四烷酰佛波醇-13-乙酸酯增强巨核细胞标志物的表达，并增加了几倍的PDGF样蛋白的分泌。与这些变化一致，在所有细胞系中均显著诱导c-sis/PDGF-B信使RNA（mRNA）表达，而一个细胞系显示显著同时诱导PDGF-A mRNA表达。PDGF样分泌是否是这些细胞系所表现的干细胞样表型的一部分，还是继发于白血病转化仍有待确定。然而，这些细胞系为研究PDGF类似物在造血细胞中的表达和功能提供了新的细胞模型。图片
We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells.Images