Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults
Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults
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DOI：
10.1016/s1386-6532(03)00002-7
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发表时间：
2003-10-01
影响因子：
8.8
通讯作者：
Falsey, AR
中科院分区：
文献类型：
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作者：
Casiano-Colón, AE;Hulbert, BB;Falsey, AR
由于老年人和成人心肺疾病患者的呼吸道合胞病毒(RSV)排泄率低，利用现有的快速抗原检测试验诊断呼吸道合胞病毒(RSV)感染可能很困难。这些测试已经在住院婴儿中进行了广泛的评估，但关于它们在成人人群中的有效性的数据有限。我们评价了三种不同的快速抗原检测方法：Becton Dickinson Directigen RSV(BD)、Bartels RSV直接荧光抗体检测(DFA)和VIDAS RSV检测(VIDAS)在60例经细胞培养、血清学或逆转录聚合酶链式反应(RT-PCR)证实为RSV感染的老年人鼻咽标本中的性能。在所有60个样本上测试的三种快速抗原检测结果都不佳。在RSV感染者中，DFA检出14例(23%)，VIDAS检出12例(20%)，BD检出6例(10%)。快速抗原检测试验对成人呼吸道合胞病毒疾病的诊断价值有限。(C)2003 Elsevier Science B.V.保留所有权利。
The diagnosis of respiratory syncytial virus (RSV) infection in older individuals and adults with cardiopulmonary diseases using available rapid antigen detection tests may be difficult due to the low virus shedding in this population. These tests have been extensively evaluated in hospitalized infants, but there is only limited data on their usefulness in adult populations. We evaluated the performance of three different rapid antigen detection tests: Becton Dickinson Directigen RSV (BD), Bartels RSV Direct Fluorescent Antibody Test (DFA) and the VIDAS RSV assay (VIDAS) in nasopharyngeal specimens from 60 older persons with RSV infection documented by cell culture, serology or reverse transcription polymerase chain reaction (RT-PCR). The three rapid antigen assays tested on all 60 samples performed poorly. DFA detected 14 (23%), VIDAS detected 12 (20%) and BD detected only 6 (10%) of the RSV infected persons. Rapid antigen detection tests for the diagnosis of RSV respiratory illness in adults are of limited value. (C) 2003 Elsevier Science B.V. All rights reserved.