Recent advances in capillary electrophoresis and capillary electrochromatography of peptides
Recent advances in capillary electrophoresis and capillary electrochromatography of peptides
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DOI：
10.1002/elps.200305660
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发表时间：
2003-12
期刊：
影响因子：
2.9
通讯作者：
V. Kašička
中科院分区：
文献类型：
--
作者：
V. Kašička
本文综述了高效毛细管电迁移方法，即区带电泳、等速电泳、等电聚焦、亲和电泳、电动色谱和电色谱在多肽分析、制备和理化表征中的应用。肽的电迁移行为的理论描述和实验验证的新方法和方法方面的毛细管电分离的肽，如合理选择的分离条件，样品处理，和抑制吸附，进行了讨论，并显示在个别分离模式和新的检测系统的设计应用于肽分离的新发展。介绍了毛细管电迁移法在肽分析中的几种应用：质量控制和纯度检测、生物基质测定、理化变化和酶促转化监测、氨基酸和序列分析以及肽图谱分析。给出了微制备肽分离的实例，并证明了毛细管电迁移技术提供肽的重要物理化学特性的能力。
An overview of the recent developments in the applications of high‐performance capillary electromigration methods, namely zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides is presented. New approaches to the theoretical description and experimental verification of the electromigration behavior of peptides and the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample treatment, and suppression of adsorption, are discussed, and new developments in individual separation modes and new designs of detection systems applied to peptide separations are shown. Several types of applications of capillary electromigration methods to peptide analysis are presented: quality control and purity tests, determination in biomatrices, monitoring of physical and chemical changes and enzymatic conversions, amino acid and sequence analysis, and peptide mapping. The examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.