Probiotic potential of Bacillus velezensis: Antimicrobial activity against non-O1 Vibrio cholerae and immune enhancement effects on Macrobrachium nipponense
Probiotic potential of Bacillus velezensis: Antimicrobial activity against non-O1 Vibrio cholerae and immune enhancement effects on Macrobrachium nipponense
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贝莱斯芽孢杆菌的益生潜力：对非 O1 霍乱弧菌的抗菌活性和对日本沼虾的免疫增强作用
DOI：
10.1016/j.aquaculture.2021.736817
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发表时间：
2021
期刊：
影响因子：
4.5
通讯作者：
Xiaojun Zhang
中科院分区：
文献类型：
--
作者：
Xinhai Zhu;Shuangmin Zhang;Liying Zhou;Shiqi Ao;Huanyu Tang;Yifan Zhou;Qiyun Chen;Xiaojian Gao;Qun Jiang;Xiaojun Zhang
本研究旨在探讨日本沼虾感染非o1型霍乱弧菌的抑菌活性和免疫调节作用。抗菌试验表明b。velezensispa1 - 1对非o1v型霍乱菌具有抑菌活性，对b型霍乱菌具有最低抑菌浓度。velezensispa1 - 1抗非o1v型霍乱发酵液为2.7 × 105CFU/mL。组胶卷暗盒。分别以105,106和107CFU/mL的b处理日本猪。分别浸泡20 d，并于5、10、15和20 d检测各免疫参数和水质指标。结果表明，在不同条件下，黄鳝的酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和超氧化物歧化酶（SOD）活性显著降低。nipponense在b中显著增加。免疫相关基因（包括crustin、Dorsal、Hemocyanim、p38、prophenoloxidase、statinm） mRNA的表达水平。经b治疗后，nipponense显著上调。velezensis。此外,theB。velezensisCPA1-1能降解培养水中的氨氮和亚硝酸盐。用非o1v型霍乱菌攻毒后，m。nipponenseinB。治疗组与对照组相比有明显差异。这些结果表明b。velezensispa1 - 1是一种潜在的益生菌，可治疗非o1型霍乱弧菌感染。nipponense。
The aim of study was to explore the antimicrobial activity and immunomodulatory effect ofBacillus velezensison non-O1Vibrio choleraeinfection inMacrobrachium nipponense. The antibacterial test showed thatB. velezensisCPA1–1 had antibacterial activity against non-O1V. cholerae, and the minimum inhibitory concentration ofB. velezensisCPA1–1 fermentation broth against non-O1V. choleraewas 2.7 × 105CFU/mL. Groups ofM. nipponensewere treated with 105, 106and 107CFU/mL ofB. velezensisby immersion bath for 20 days, respectively, and various immune parameters and water quality indexes were examined at 5, 10, 15 and 20 d. Results indicated that the acid phosphatase (ACP), alkaline phosphatase (AKP), and superoxide dismutase (SOD) activity ofM. nipponensewere increased significantly inB. velezensistreated group, and the mRNA expression level of immune-related genes includingCrustin,Dorsal, Hemocyanim,p38,ProphenoloxidaseandStatinM. nipponensewas significantly up-regulated after treating withB. velezensis. Furthermore, theB. velezensisCPA1–1 could degrade ammonia nitrogen and nitrite in culture water. After challenge with non-O1V. cholerae, it is higher of the survival rate ofM. nipponenseinB. velezensistreated group than that of the control group. These results indicate thatB. velezensisCPA1–1 is a potential probiotic against non-O1Vibrio choleraeinfection inM. nipponense.