Accumulation of polyunsaturated fatty acid-derived metabolites in the sarcopenic muscle of aging mice
Accumulation of polyunsaturated fatty acid-derived metabolites in the sarcopenic muscle of aging mice
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DOI：
10.1111/ggi.14561
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发表时间：
2023-02-22
影响因子：
3.3
通讯作者：
Daida，Hiroyuki
中科院分区：
文献类型：
--
作者：
Kadoguchi，Tomoyasu;Shimada，Kazunori;Daida，Hiroyuki
目的虽然已知高龄会改变骨骼肌脂质代谢，但多不饱和脂肪酸衍生代谢物（主要是类二十烷酸和二十二烷酸）在肌肉减少症中的作用尚不清楚。因此，我们研究的变化，花生四烯酸，二十碳五烯酸和二十二碳六烯酸的代谢产物中的sarcoopenic muscle of aged mice.MethodsWe使用6和24个月大的雄性C57 BL/6 J小鼠作为健康和sarcoopenic muscle模型，分别。从下肢的骨骼肌，并进行了液相色谱-串联质谱analysis.ResultsThe液相色谱-串联质谱分析检测到老年小鼠的肌肉中的代谢产物的明显变化。在鉴定的63种代谢物中，与年轻小鼠的健康肌肉相比，9种在老年小鼠的肌肉减少中显著更高。特别地，前列腺素E2、前列腺素F2 a、血栓烷B2、5-羟基二十碳四烯酸和15-氧代二十碳四烯酸（花生四烯酸衍生代谢物），12-羟基-二十碳五烯酸和14，15-环氧-二十碳四烯酸（二十碳五烯酸衍生代谢物）和10-羟基二十二碳六烯酸和14-羟基十八碳五烯酸结论老年小鼠肌肉中存在代谢产物的积累。我们的研究结果可能为衰老或疾病相关的肌肉减少症的发病机制和进展提供新的见解。Geriatr Gerontol Int 2023; 23：297-303。
AimAlthough it is known that advanced age alters skeletal muscle lipid metabolism, the role(s) of polyunsaturated fatty acid‐derived metabolites (mostly eicosanoids and docosanoids) in sarcopenia are not clear. We therefore examined the changes in the metabolites of arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid in the sarcopenic muscle of aged mice.MethodsWe used 6‐ and 24‐month‐old male C57BL/6J mice as healthy and sarcopenic muscle models, respectively. Skeletal muscles were removed from the lower limb and subjected to a liquid chromatography–tandem mass spectrometry analysis.ResultsThe liquid chromatography–tandem mass spectrometry analysis detected distinct changes of metabolites in the muscles of the aged mice. Of the 63 metabolites identified, nine were significantly higher in the sarcopenic muscle of aged mice compared with the healthy muscle of young mice. In particular, prostaglandin E2, prostaglandin F2a, thromboxane B2, 5‐hydroxyeicosatetraenoic acid, and 15‐oxo‐eicosatetraenoic acid (arachidonic acid‐derived metabolites), 12‐hydroxy‐eicosapentaenoic acid and 14,15‐epoxy‐eicosatetraenoic acid (eicosapentaenoic acid‐derived metabolites) and 10‐hydroxydocosa‐hexaenoic acid and 14‐hydroxyoctadeca‐pentaenoic acid (docosahexaenoic acid‐derived metabolites) were significantly higher in aged tissue compared with young tissue (allP< 0.05).ConclusionsWe observed the accumulation of metabolites in the sarcopenic muscle of aged mice. Our results may provide new insights into the pathogenesis and progression of aging‐ or disease‐related sarcopenia.Geriatr Gerontol Int 2023; 23: 297–303.