HLA profile in patients with AIDS and tuberculosis
HLA profile in patients with AIDS and tuberculosis
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DOI：
10.1590/s1413-86702008000400004
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发表时间：
2008-08-01
影响因子：
3.4
通讯作者：
Donadi, Eduardo Antonio
中科院分区：
文献类型：
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作者：
Figueiredo, José Fernando de Castro;Rodrigues, Maria de Lourdes Veronese;Donadi, Eduardo Antonio
在不同人群中进行的研究报告了某些HLA特异性与结核病易感性之间的关联。我们调查了不同种族背景的巴西艾滋病和结核病患者的I类和II类HLA谱。对22名成年艾滋病合并结核病患者（第一组）、103名无结核病艾滋病患者（第二组）和423名未感染艾滋病毒的健康个体（第三组）进行了评估。ELISA法诊断HIV感染，明胶颗粒凝集试验证实。结核病的诊断是基于临床/放射学表现和直接杆菌镜检查或临床标本培养。用微淋巴毒性分型ⅰ类抗原。II类等位基因采用聚合酶链反应（PCR）进行鉴定。组间HLA特异性频率的差异见于以下抗原/等位基因：I组x II组：HLA- a31 - p=0.026；HLA-B41 - p=0.037；HLA-DRB1 * 10 p = 0.037;HLA- DQB1*5 - p=0.009。ⅰ组xⅲ组（对照）：HLA-A31 - p=0.000008；优势比(OR)=31.75；HLA-B41 - p=0.003；HLA-DQB1*5 - p=0.02。HLA- a31和HLA- b41抗原以及HLA- drb1 *10和HLA- dqb1 *05等位基因在艾滋病和结核病患者中被过度代表（I组），这表明这些HLA分子与巴西艾滋病患者对结核病的易感性有关。
Studies carried out in various populations have reported an association between some HLA specificities and susceptibility to tuberculosis. We investigated the class I and class II HLA profile in Brazilian patients of various ethnic backgrounds who had AIDS and tuberculosis. Twenty-two adult patients with AIDS and tuberculosis (Group I), 103 patients with AIDS without tuberculosis (Group II) and 423 healthy individuals not infected with HIV (Group III) were evaluated. Diagnosis of HIV infection was made by ELISA, confirmed by a gelatin particle agglutination test. Diagnosis of tuberculosis was made based on clinical/radiological presentation and direct bacilloscopy or clinical specimen cultures. Class I antigens were typed by microlymphotoxicity. Class II alleles were characterized by the polymerase chain reaction (PCR). Differences in frequency of HLA specificities between groups were found in the following antigens/alleles: Group I x Group II: HLA-A31 - p=0.026; HLA-B41 - p=0.037; HLA-DRB1*10 p=0.037; HLA- DQB1*5 - p=0.009. Group I x Group III (control): HLA-A31 - p=0.000008; odds ratio (OR)=31.75; HLA-B41 - p=0.003; HLA-DQB1*5 - p=0.02. HLA-A31 and HLA-B41 antigens and the HLA-DRB1*10 and HLA-DQB1*05 alleles were over-represented in patients with AIDS and tuberculosis (Group I), suggesting that these HLA molecules are associated with susceptibility to tuberculosis in Brazilian patients with AIDS.