Selective in vitro reversal of the insulin resistance of glucose transport in denervated rat skeletal muscle.
Selective in vitro reversal of the insulin resistance of glucose transport in denervated rat skeletal muscle.
复制标题
体外选择性逆转去神经大鼠骨骼肌中葡萄糖转运的胰岛素抵抗。
DOI：
10.1152/ajpendo.1989.257.3.e418
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Buse,MG
中科院分区：
文献类型：
--
作者：
Sowell,MO;Dutton,SL;Buse,MG
骨骼肌去神经支配（24小时）导致葡萄糖运输和糖原合成的严重受体后胰岛素抵抗，这在短时间（30分钟）预孵育后的孤立肌肉中可以证明。经过较长时间的预孵育（2-4小时），葡萄糖转运的胰岛素反应增加到正常，而糖原合成仍保持胰岛素抵抗。在短时间或长时间的孵育后，去神经支配肌肉的基础和胰岛素刺激的氨基酸运输明显低于对照组，尽管胰岛素刺激的运输百分比没有显著差异。去神经支配后葡萄糖转运胰岛素抵抗的发展不能归因于对糖皮质激素或腺苷的敏感性增加。葡萄糖转运胰岛素抵抗的选择性体外逆转不依赖于培养基成分，不需要蛋白质或前列腺素合成，也不能归因于向培养基中释放阳性调节因子。这些数据表明：1)肌肉中的胰岛素受体通过其他胰岛素敏感效应系统所不具有的信号通路刺激葡萄糖转运；2)去神经支配可能在多个位点影响胰岛素受体信号转导。
Denervation (24 h) of skeletal muscle causes severe postreceptor insulin resistance of glucose transport and glycogen synthesis that is demonstrable in isolated muscles after short (30 min) preincubations. After longer preincubations (2-4 h), the insulin response of glucose transport increased to normal, whereas glycogen synthesis remained insulin resistant. Basal and insulin-stimulated amino acid transport were significantly lower in denervated muscles than in controls after short or long incubations, although the percentage stimulation of transport by insulin was not significantly different. The development of glucose transport insulin resistance after denervation was not attributable to increased sensitivity to glucocorticoids or adenosine. The selective in vitro reversal of glucose transport insulin resistance was not dependent on medium composition, did not require protein or prostaglandin synthesis, and could not be attributed to release of a positive regulator into the medium. The data suggest 1) the insulin receptor in muscle stimulates glucose transport by a signaling pathway that is not shared by other insulin-sensitive effector systems, and 2) denervation may affect insulin receptor signal transduction at more than one site.