Breviscapine-induced Apoptosis of Human Hepatocellular Carcinoma Cell Line HepG2 was Involved in its Antitumor Activity
Breviscapine-induced Apoptosis of Human Hepatocellular Carcinoma Cell Line HepG2 was Involved in its Antitumor Activity
复制标题
布维司卡平诱导人肝细胞癌细胞系 HepG2 的凋亡与其抗肿瘤活性有关
DOI：
10.1002/ptr.3002
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发表时间：
2010-08-01
影响因子：
7.2
通讯作者：
Guo, Qinglong
中科院分区：
文献类型：
--
作者：
Wu, Yulin;Fan, Qianqian;Guo, Qinglong
灯盏花素是从传统中药灯盏细辛中分离出来的黄酮类成分。目的探讨灯盏花素对人肝癌细胞株HepG2的诱导凋亡作用并探讨相关分子机制。不同浓度灯盏花素处理HepG2细胞，MTT法分析抑制率。倒置光学显微镜和荧光显微镜观察细胞形态变化，流式细胞术检测细胞凋亡率。 Western blot用于评估蛋白表达。 HepG2细胞的活力以浓度依赖性方式被显着抑制，并且证实了明显的形态学变化，包括染色质浓缩和体积减少。流式细胞仪显示凋亡细胞百分比增加，蛋白质印迹检测多种凋亡相关蛋白 Bcl-2、Bax 和 caspase-3 表达水平的改变。首次发现灯盏花素具有潜在的抗肿瘤活性，可诱导 HepG2 细胞显着凋亡，有望成为未来癌症治疗的新候选者。版权所有 (C) 2010 约翰·威利父子有限公司
Breviscapine is a flavonoid constituent isolated from a traditional Chinese herb Erigerin breviscapus (Vant.) Hand-Mazz. To investigate the apoptosis-inducing effect of breviscapine on human hepatocellular carcinoma cell line HepG2 and explore the relative molecular mechanisms. HepG2 cells were treated with breviscapine at different concentrations and the inhibitory rate was analyzed by MTT assay. The morphological changes in cells were observed under an inverted light microscope and a fluorescence microscope and the apoptosis rate were detected by flow cytometry. Western blot was used to evaluate the protein expression. The viability of HepG2 cells was markedly inhibited in a concentration-dependent manner and obvious morphological changes were confirmed, including condensed chromatin and reduction in volume. The increased percentage of apoptotic cells was displayed by flow cytometry and the altered expression level of several apoptosis-associated proteins, Bcl-2, Bax and caspase-3, was detected by western blot. It is first discovered that breviscapine exhibited potential antitumor activity, induces remarkable apoptosis in HepG2 cells and promises to be a new candidate in future cancer therapy. Copyright (C) 2010 John Wiley & Sons, Ltd.