Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging.
Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging.
复制标题
超分辨率显微镜：为体内成像提供新思路
DOI：
10.3389/fchem.2021.746900
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
Qu J
中科院分区：
文献类型：
--
作者：
Jing Y;Zhang C;Yu B;Lin D;Qu J
在过去的二十年里，超分辨率显微镜(SRM)在分辨率方面比传统的光学显微镜有了显著的提高，已经成为显示固定细胞和活细胞中生物活动的有力工具。然而，完全理解生物过程需要在高时空分辨率的生理背景下研究细胞。最近，SRM已经展示了它观察活物种的详细结构和动力学的能力。在这里，我们总结了SRM的最新技术进展，这些技术已经成功地应用于活体成像。然后，讨论了标记策略的改进，以及对荧光团的光谱和化学要求。最后，我们广泛地回顾了当前超分辨技术在活体物种中的应用，并强调了这一新兴领域面临的一些内在挑战。我们希望这篇综述能为体内超分辨率成像相关领域的研究人员和初学者提供理想的参考。
Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to in vivo imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of in vivo super resolution imaging.