New class of IMP cyclohydrolases in Methanococcus jannaschii
New class of IMP cyclohydrolases in Methanococcus jannaschii
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DOI：
10.1128/jb.184.5.1471-1473.2002
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发表时间：
2002-03-01
影响因子：
3.2
通讯作者：
White, RH
中科院分区：
文献类型：
--
作者：
Graupner, M;Xu, HM;White, RH
对jannaschii甲烷球菌中IMP环水解酶活性的酶进行了纯化和测序，发现其基因座对应于MJ0626基因。MJ0626基因克隆成功，其蛋白产物在大肠杆菌中表达，可催化5-甲酰基酰胺咪唑-4-羧酰胺核糖核苷酸环化为IMP，该酶与已知酶序列无相似性，其催化性能与已知的IMP环水解酶不同。该酶的purO基因目前仅在古细菌域中发现。
The enzyme responsible for observed IMP cyclohydrolase activity in Methanococcus jannaschii was purified and sequenced: its genetic locus was found to correspond to gene MJ0626. The MJ0626 gene was cloned, and its protein product was expressed in Escherichia coli and shown to catalyze the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP. The enzyme has no sequence similarity to known enzymes, and its catalytic properties appear distinct from any characterized IMP cyclohydrolase. The purO gene for the enzyme is currently found only in the domain Archaea.