Differential susceptibility of bone marrow-derived dendritic cells and macrophages to productive infection with Listeria monocytogenes.
Differential susceptibility of bone marrow-derived dendritic cells and macrophages to productive infection with Listeria monocytogenes.
复制标题
骨髓来源的树突状细胞和巨噬细胞对单核细胞增生李斯特氏菌的生产性感染的敏感性不同。
DOI：
10.1111/j.1462-5822.2006.00880.x
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发表时间：
2007
影响因子：
3.4
通讯作者：
Hiltbold,ElizabethM
中科院分区：
文献类型：
--
作者：
Westcott,MarlenaM;Henry,CurtisJ;Cook,AnneS;Grant,KennethW;Hiltbold,ElizabethM
树突状细胞（DC）是抗单核细胞增生李斯特菌免疫应答所必需的细胞，在体内和体外均可被感染。然而，目前尚不清楚DC是否为细菌生长提供了理想的细胞内生态位。为了解决这个问题，我们比较了单核细胞增生李斯特菌在小鼠骨髓来源的DC和巨噬细胞（BMM）中的行为。 与BMM类似，细菌逃逸到DC中的胞质溶胶中，复制并扩散到相邻细胞。然而，DC感染在细胞内倍增时间和细菌数量的总增加方面不太稳健。免疫荧光分析使用一株单核细胞增生李斯特菌，表达绿色荧光蛋白后，细菌进入细胞质表明，一个亚群的DC限制细菌的空泡，这一发现证实了电子显微镜。 在未经刺激的DC培养物中，单核细胞增生李斯特菌优先在表型不成熟的细胞中复制。 此外，在感染前诱导成熟的DC是细菌生长的不良宿主。我们得出结论，DC为单核细胞增生李斯特菌的生长提供了一个次优的生态位，这至少部分是DC成熟状态的函数。 因此，有效T细胞应答的产生可能是DC的生产性和非生产性感染的净效应。
Dendritic cells (DC) are required for the immune response againstListeria monocytogenesand are permissive for infectionin vivoandin vitro. However, it is unclear if DC provide a desirable intracellular niche for bacterial growth. To address this issue, we have compared the behaviour ofL. monocytogenesin murine bone marrow‐derived DC and macrophages (BMM). Similar to BMM, bacteria escaped to the cytosol in DC, replicated, and spread to adjacent cells. However, DC infection was less robust in terms of intracellular doubling time and total increase in bacterial numbers. Immunofluorescence analysis using a strain ofL. monocytogenesthat expresses green fluorescent protein upon bacterial entry into the cytosol suggested that a subpopulation of DC restricted bacteria to vacuoles, a finding that was confirmed by electron microscopy. In unstimulated DC cultures,L. monocytogenesreplicated preferentially in phenotypically immature cells. Furthermore, DC that were induced to mature prior to infection were poor hosts for bacterial growth. We conclude that DC provide a suboptimal niche forL. monocytogenesgrowth, and this is at least in part a function of the DC maturation state. Therefore, the generation of an effective T cell response may be a net effect of both productive and non‐productive infection of DC.