Plasmonic Fluor-Enhanced Antigen Arrays for High-Throughput, Serological Studies of SARS-CoV-2.
Plasmonic Fluor-Enhanced Antigen Arrays for High-Throughput, Serological Studies of SARS-CoV-2.
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DOI：
10.1021/acsinfecdis.2c00086
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发表时间：
2022-08-12
影响因子：
5.3
通讯作者：
Amarasinghe, Gaya K.
中科院分区：
文献类型：
--
作者：
Qavi, Abraham J.;Wu, Chao;Lloyd, Matthew;Zaman, Mohammad Mahabub-Uz;Luan, Jingyi;Ballman, Claire;Leung, Daisy W.;Crick, Scott L.;Farnsworth, Christopher W.;Amarasinghe, Gaya K.
急性感染或既往暴露的血清学检测对于疫情期间的患者管理和公共卫生决策协调至关重要。目前的方法有几个局限性，包括可变的性能，相对较低的分析和临床灵敏度，以及由于抗原漂移检测不良。针对持续的COVID-19大流行的SARS-CoV-2检测的血清学方法受到其中几个限制，并提醒人们迫切需要新技术。在这里，我们描述了使用超亮荧光试剂，Plasmonic Fluors，再加上抗原阵列，解决了这些限制的一个子集。我们证明了其应用程序中使用的SARS-CoV-2血清学检测的患者样本。在我们的多重检测中，将SARS-CoV-2抗原点样到96孔板的单个孔内的48重阵列中，并用于评估SARS-CoV-2阳性患者的剩余实验室样本。用Auragent Bioscience的Empower酶标仪和微阵列分析软件进行信号读出。使用1 μL样品体积。Plasmonic Fluors的高灵敏度与阵列形式相结合，使我们能够分析患者对八种不同SARS-CoV-2抗原的血清学反应，并评估对IgG，IgM和伊加的反应。在症状状态期间，SARS-CoV-2抗原的敏感性在72.5%和95.0%之间，特异性在62.5%和100%之间，并且由此产生的曲线下面积值在0.76和0.97之间。总之，这些结果突出了低样品体积和多重能力的灵敏度增加。这些特征使得等离子体荧光增强抗原阵列成为一种有吸引力的技术，用于COVID-19大流行及其他疾病的血清学研究。
Serological testing for acute infection or prior exposure is critical for patient management and coordination of public health decisions during outbreaks. Current methods have several limitations, including variable performance, relatively low analytical and clinical sensitivity, and poor detection due to antigenic drift. Serological methods for SARS-CoV-2 detection for the ongoing COVID-19 pandemic suffer from several of these limitations and serves as a reminder of the critical need for new technologies. Here, we describe the use of ultrabright fluorescent reagents, Plasmonic Fluors, coupled with antigen arrays that address a subset of these limitations. We demonstrate its application using patient samples in SARS-CoV-2 serological assays. In our multiplexed assay, SARS-CoV-2 antigens were spotted into 48-plex arrays within a single well of a 96-well plate and used to evaluate remnant laboratory samples of SARS-CoV-2 positive patients. Signal-readout was performed with Auragent Bioscience’s Empower microplate reader, and microarray analysis software. Sample volumes of 1 μL were used. High sensitivity of the Plasmonic Fluors combined with the array format enabled us to profile patient serological response to eight distinct SARS-CoV-2 antigens and evaluate responses to IgG, IgM, and IgA. Sensitivities for SARS-CoV-2 antigens during the symptomatic state ranged between 72.5 and 95.0%, specificity between 62.5 and 100%, and the resulting area under the curve values between 0.76 and 0.97. Together, these results highlight the increased sensitivity for low sample volumes and multiplex capability. These characteristics make Plasmonic Fluor-enhanced antigen arrays an attractive technology for serological studies for the COVID-19 pandemic and beyond.
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影响因子：
4
作者：
Hui, Yuen Yung;Lu, Yu-Chun;Chang, Huan-Cheng
通讯作者：
Chang, Huan-Cheng
DOI：
10.1021/jz201147g
发表时间：
2011-11-17
期刊：
The journal of physical chemistry letters
影响因子：
--
作者：
Kemling JW;Qavi AJ;Bailey RC;Suslick KS
通讯作者：
Suslick KS
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F
DOI：
10.1172/jci142004
发表时间：
2020-11-02
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Klein, Sabra L;Pekosz, Andrew;Tobian, Aaron Ar
通讯作者：
Tobian, Aaron Ar
影响因子：
30.5
作者：
Hachim, Asmaa;Kavian, Niloufar;Valkenburg, Sophie A.
通讯作者：
Valkenburg, Sophie A.