Cortical Representations Are Reinstated by the Hippocampus during Memory Retrieval
Cortical Representations Are Reinstated by the Hippocampus during Memory Retrieval
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DOI：
10.1016/j.neuron.2014.09.037
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发表时间：
2014-10-22
期刊：
影响因子：
16.2
通讯作者：
Wiltgen, Brian J.
中科院分区：
文献类型：
--
作者：
Tanaka, Kazumasa Z.;Pevzner, Aleksandr;Wiltgen, Brian J.
海马体被认为通过恢复学习期间观察到的皮质活动模式来检索记忆。为了验证这一想法，我们监测了单个皮层神经元的活动，同时使海马体失活。在情境恐惧条件反射过程中活跃的神经元被标记有持久的荧光蛋白H2 B-GFP和光激活的质子泵ArchT。这些蛋白质使我们能够在学习几天后识别编码神经元，并通过激光刺激使它们沉默。当标记的CA 1细胞被沉默，我们发现，记忆提取受损，在皮层（内嗅，压后，嗅周）和杏仁核的代表不能被重新激活。重要的是，海马失活并没有改变大多数大脑区域的活动总量。相反，它选择性地阻止了在学习过程中活跃的神经元在提取过程中被重新激活。这些数据提供了海马体在提取过程中重新激活特定记忆表征的功能证据。
The hippocampus is assumed to retrieve memory by reinstating patterns of cortical activity that were observed during learning. To test this idea, we monitored the activity of individual cortical neurons while simultaneously inactivating the hippocampus. Neurons that were active during context fear conditioning were tagged with the long-lasting fluorescent protein H2B-GFP and the light-activated proton pump ArchT. These proteins allowed us to identify encoding neurons several days after learning and silence them with laser stimulation. When tagged CA1 cells were silenced, we found that memory retrieval was impaired and representations in the cortex (entorhinal, retrosplenial, perirhinal) and the amygdala could not be reactivated. Importantly, hippocampal inactivation did not alter the total amount of activity in most brain regions. Instead, it selectively prevented neurons that were active during learning from being reactivated during retrieval. These data provide functional evidence that the hippocampus reactivates specific memory representations during retrieval.